Hi,
I would like to extract triple helix Schizophyllan from the fungus Schizophyllum commune (dried). From my preliminary research, I devised a method to extract dried powder in distilled water (2x 90 C; 1 h), then extract again in 0.1 M NaOH (2x 60C; 1 h), combined supernatants, filter, neutralise with HOCl and precipitate with 3 volumes of 95% ethanol. The precipitate then dried and weighed.
From my research, it seems hot water extraction, alkaline and solvent will denature the triple helix structure into single helix or random coiled structures.
Is there another approach for extraction to preserve the triple helix beta glucan? Can the extracted structures renature into the triple helix structures?
Many thanks in advance