I have cultured fungus which produces honey like substance after five days of growth. I want to know what are these substances. How can I isolate the substances produced?
Please contact: Dr. Tulasi Satyanarayana at .
or Prof. Dr. Ramesh Kuhad at
They may be able to answer.
In the context of Electron Spin Resonance (ESR) spectroscopy, under what conditions is an absorption spectrum considered to be a single absorption spectrum?
25 July 2024 2,325 1 View
I am doing a resazurin assay to determine the MIC of plant extracts against Mycobacterium tuberculosis. The problem is the MIC is coming at a very high value. I read in one paper that even if...
01 January 2024 7,916 2 View
I have some single channel EEG data which are in the .csv format. How to import this .csv File to EEGlab and analyze them? Or is there any way to convert these data to .edf or .bdf format (Biosemi...
07 September 2023 1,200 1 View
Please explain about the IRF (Instrument Response Function)? How it is measured and why it is important?
23 January 2023 1,700 2 View
I am trying to detect a plant compound Asiatic acid.In literature, the detection is very low 205 nm. When I try at this wavelength, there is huge noise and a baseline shift. I am using...
07 June 2022 9,394 0 View
I am looking for some good critics on Bourdieu's Rules of Art.
06 February 2022 6,458 2 View
I am doing TPC assay. I obtained the equation y=0.0006x -0.0123 . I started with 8g of dry plant powder for extraction and dissolved it in 80 ml of methanol. I evaporated the total 80 ml volume...
21 November 2021 2,778 0 View
I want to extract quercetin from the leaves of Centella asiatica. It is an aglycone flavonoid and therefore is less polar compared to other flavonoids . What should be the appropriate solvent -...
14 October 2021 3,968 6 View
I have taken 1 gm of dry leaf powder ad extracted it in 10 ml of methanol solvent.I got crude extract of 70 mg.I did the AlCl3 assay for total flavonoid content for 2 mg / ml . I got the value of...
17 September 2021 644 2 View
I have air dried and freeze dried my leaf sample .After grinding can I store it in a cool and dark place at room temperature until analysis....?
07 August 2021 1,016 3 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View