A biocatalytic hydrogenation of carboxylic acids

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DdPCR analysis: biological replicates ?

--Hi, such as RTqPCR to analyse gene expression, is it necessary to have biological replicates in a ddPCR experiment ? thank yu --

21 May 2024 8,181 6 View

Model for reconstructing the number of inhabitants from the number of burials ?

hi, I'm trying to find out if there are any models for the medieval period south of the Sahara in West Africa that can be used to reconstitute the number of inhabitants at a site from the number...

04 November 2023 2,666 1 View

Aerosol biocontamination and sanitation network ?

Good morning, I am looking for publications/data regarding aerosol biocontamination from the sanitation network lift stations. Would you be aware of any articles or complications on this...

17 July 2023 2,369 0 View

Freeze Drying small volumes : What should be considered ?

Hi All, we usually discuss different tips in order to freeze dry large volume samples (e.g. two steps freezing, etc). I would like to ask you if you have any tips regarding the small volumes ? For...

20 June 2023 4,324 2 View

What do we know about the costs of synthetic fuels in road transport?

From 2035 on, besides fully electric cars, the EU will also allow the putting on the market of cars powered by synthetic fuels/e-fuels. Do you know an up-to-date, reliable independent estimate of...

29 March 2023 9,160 2 View

What is the significance difference between a p-value of 0.01 and a p-value of 0.012 ?

--Hi, p-value = 0.01 and p-value = 0.012 is it risky to say that it is the same thing in terms of probability ? p=0.01 --> * * p=0.012 (p>0.01) --> * Thank you --

09 November 2022 7,448 3 View

How to sample blood on X. laevis ?

Hi everyone, I am currently working with X. laevis and I would like to collect blood samples. This would happened right before euthanasia on anesthetized individuals. My aim is to measure...

20 June 2022 8,691 0 View

Could you recommend a method or pipeline for quality control and paralog removal from amplicon sequencing data?

Hello everyone, I have recently started working with NGS data (amplicon sequencing) for phylogenetics and species-delimitation studies of closely-related specimens (typically within a genus or a...

02 April 2022 3,139 0 View

Are changes significant?

Dear everyone, I've seen many posts in this community that seem to answer my questions but I have a tough time figuring out how my situation compares to the one described by the op's and I'm...

24 December 2021 7,852 4 View

Who can tell me how to employ an Endogenous-Switching Poisson regression model ?

I want to employ Endogenous-Switching Poisson regression model to determine the impact of x on Y. However, I don't know the succession of the variables from to the command , dependent variables ,...

06 September 2021 8,159 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,938 1 View

How to eliminate the carry-over effect in GC-MS/MS system?

Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...

22 July 2024 5,492 6 View