Study Design for 3 Interval DVs, mixed IVs?

I'm conducting a study on mental health terminology on social media for a group project. We have three interval DVs measured through likert scales and three IVs. The IVs are social media usage...

28 January 2021 2,871 3 View

What is the best Perception instrument that I could use for Student Perception towards a subject?

I am starting to write my thesis for Graduate School and I am struggling to find appropriate instrument regarding student perception instrument. The perceptions to be discussed are (a) interest...

17 January 2021 9,218 3 View

Alpha Reliability for dichotomous nominal data?

I am trying to run alpha reliability tests in SPSS on 4 questionnaires I have used. Questionnaires which have ordinal data/items is fine but I have questionnaires/questions/scales in which the...

18 August 2020 8,426 6 View

How to run longitudinal mediation with multiple (5 waves)?

Hi everyone! I'm doing a PhD in Clinical Psychology and I have some treatment data to analyse. The design is a 2 (condition: self-compassion, cognitive restructuring) x 5 (time: baseline,...

01 August 2018 4,502 3 View

When extracting peak currents in Cyclic Voltammetry, what processes does the baseline represent?

I know that when extracting the peak anodic/cathodic currents, it is typical to extract a baseline. I presume to account for non-faradaic processes (i.e. double layer charging), however I have not...

08 January 2018 3,824 7 View

What does a peak current separation of >59 mV in a CV plot for a supposed reversible couple mean?

04 April 2017 726 7 View

Why use a 3 electrode set-up for non-faradaic measurements?

What are the arguments of making a distinction between the CE and RE if I am measuring non-faradaic processes (i.e. binding events to a surface modified WE)? I understand the arguments for...

30 March 2017 3,262 3 View

How does choice of Reference Electrode material affect the observed equilibrium potential of a redox couple?

Hello, so this is something that has been bothering me, and I can't seem to find the answer. I am running characterization CV experiments using the redox couple ferri-/ferrocyanide. My working and...

28 February 2017 8,868 1 View

How can I open and read a .tsf file containing raman spectrum?

I have a series of raman spectra sent to me by a collaborator to perform some data analysis on. The data are .tsf files which I have not dealt with before and I can't find how to read these files...

08 February 2014 1,964 3 View

Using sodium ion adducts in LC/MS for quantification of steroids?

I am interested in quantifying a range of steroids by LC/MS (C18 column with 70% methanol and 30% 25 mM ammonium acetate mobile phase, using atmospheric pressure electrospray ionisation) and...

28 July 2013 3,084 25 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View