My p.21 mice have started developing seizures after intracranial injection of 4ul AAV to the left lateral ventricle. I’ve done ~22 of these procedures already with no issues. I’m using a 24G Hamilton to inject - which I’m aware is a large needle size but I’ve never had issues before.
Is anyone able to advise what might be causing these seizures? thanks
How soon after AAV injection do you notice the seizures? While the mice are still anesthetized, or during recovery?
4 ul sounds like a lot to me - that's more than 10% of total CSF in a mouse (as far as I know). Could it be that you are generating too much intracranial pressure? I'm not familiar with ventricular injections, though, so perhaps that is a normal volume? For brain injections typical AAV volumes are ~50-500 nanoliters.
Admittedly, if you haven't changed anything and previously you didn't run into any issues with the same procedure, same virus, and same mouse strain, then this seems odd.
I recommend you ask your staff veterinarian for advice; this type of adverse outcome likely needs to be reported to them anyhow.
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