Hi,
I wonder if people know 3'deoxy ribonucleotide (i.e. with 2'OH) is commercially available? I didn't find any in the modified bases product section in IDT, thermo, or sigma. I'd like to use this to shut down elongating RNA polymerase in vitro instantaneously without denaturing it, just like how ddNTPs work for shutting down DNA polymerase in Sanger sequencing . Would be very appreciated for suggestions.
Chun-Chen
Try ThermoFisher Scientific. If they don't have it in catalogue contact the sale team
You may also check Sangon biotech company website https://www.sangon.com/. (It is the largest DNA/RNA synthesizing company in mainland China). I guess you are a Chinese too. So you can inquire them by email.
Some 3'deoxynucleosides (not nucleotides) are available from Berry & Associates:
http://www.berryassoc.com/display.asp?categoryid=15&results=display.asp%3F&pagefrom=fresh
Do you need the 3'-dNTPs? If 3'deoxynucleosides are available , it is very easy to make the 3'-dNTPs.
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