3D reconstruction of dendrites and spines, and quantification of GFP labelled cells?

06 September 2024 0 5K Report

Hi everyone,

I am working on coronal sections of rat brain (hippocampus) and I want to do 3D reconstruction of dendritic spines in the images (from confocal microscope). I would like to know the optimal thickness of the section suitable for 3D reconstruction?

Also, for the quantification of cells labelled with GFP, which plug-in in ImageJ will be BEST and EASIER to use?

Thanks in-advance

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