Aamra Mahboob

3 Questions 4 Answers 0 Followers

Questions related from Aamra Mahboob

3D reconstruction of dendrites and spines, and quantification of GFP labelled cells?

Hi everyone, I am working on coronal sections of rat brain (hippocampus) and I want to do 3D reconstruction of dendritic spines in the images (from confocal microscope). I would like to know the...

06 September 2024 4,795 0 View

Suitable size of the cannula for intrahippocampal injection in rats?

Hello everyone, I am using Sprague Dawley rats for viral vector injection into the hippocampus, using a Hamilton syringe (10 microlitre.) attached to a PE tubing that has a cannula attached at...

21 May 2024 8,811 0 View

Hello everyone, I want to detect a protein 8.9kDa using western blot technique, from Dentate Gyrus of rat brain?

Please suggest if 15% gel for SDS-PAGE would be suitable or any other option? Secondly, for transfer, a nitrocellulose membrane 0.2um or PVDF? Any other tried and tested strategies will be...

20 January 2023 2,015 5 View