How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to separate large amounts of two Riboflavin-based compounds with similar solubilities?

I'm running a reaction in where riboflavin is derivatized with a crosslinker in DMSO. The final reaction mixture contains riboflavin (non reacted) and riboflavin-crosslinker compound (slightly...

13 December 2020 4,865 3 View

Has anyone tried separating cis and trans isomers of fatty acids derivatized as their trimethylsilyl esters using N,O-BSTFA reagent on FAME columns?

I have not achieved separation of cis and trans fatty acids derivatized as trimethylsilyl esters using N,O -BSTFA on the DB-5 Column, but I prefer this derivatization method as it is rather...

18 November 2020 4,993 3 View

Can we perform MOX/MSTFA derivatization in Eppendorf tubes?

Hi there! Recently I am planning to work on metabolomics using GC-MS, where derivatization is a crucial step. Here, I am wondering if that possible to derivatize extract samples with both MOX and...

24 September 2020 9,695 1 View

How to oxidize Cysteamine containing peptides to its disulfide while cleaving them from the resin?

Dear everyone, I synthesize peptides with a common Fmoc-strategy starting from a Cysteamine 2-chlorotrityl resin. That works fine and usually I cleave the peptide from the resin with a mixture...

24 September 2020 7,907 2 View

Stagetips for desalting small molecules (not peptides)?

I work with derivatized neurotransmitter molecules. I was using C-18 SPE cartridges previously for desalting them before LC-MS, but now I would like to adopt in-house made stagetips. These are a...

22 September 2020 4,854 6 View

In grignard reaction, how can I attach 'Mg' to only one side of dibromoalkane?

As you can see in the image file, I want to make Br-C12-dithiocarboxylic acid form. However, I don't know how to control the amounts of reactant. I tried 'Mg:alkane=1:1 equiv' but many byproducts...

18 September 2020 3,970 4 View

How stable is 1-Dodecanethiol?

Hello, I am doing linearity test of 1-Dodecanethiol on GC by prepping 6 standard solutions from 10 to 35 ppm in DCM (adding BSTFS as a derivatizing agent). 1-Dodecanethiol peak comes sharp...

06 May 2020 4,287 5 View

Recommendations please - contract analytical services on protein characterization and purification?

Currently working on the nutritional profile of the seed proteins of Hyptis spicigera as a global public good, I am looking for the following analytical services: 1. extraction the total seed...

03 March 2020 357 3 View

I would like to protect my thiol group in 4-mercaptobenzoic acid molecule. Any good suggestions?

Hello All, I tried to protect the free thiol group using 4-methoxybenzyl chloride via SN2 reaction, but I was not able to do so. I am wondering if you have any suggestions on how to proceed this...

01 February 2020 3,152 5 View

Non-consistent precursor ions in LC-MS/MS and reduced intensity after derivatization using TMS-DAM (peptide mass spec)?

Hi, I am trying to develop an assay to detect a conjugated peptide in plasma. I am essentially brand new to MS but have LC experience. The analyte of interest is a bisphosphonate tethered to...

12 January 2020 3,078 1 View