I need to derivatize amino acids from a plant based beverage sample, what is the best derivatization method and the internal standard to be used while running HPLC?
use alpha-aminobutyric acid (ABU) as internal standard for amino acid analysis via HPLC. You may also use norvaline or sarcosine as internal Standards.
derivatization of amino acid with chiral Marfey's reagent.
use alpha-aminobutyric acid (ABU) as internal standard for amino acid analysis via HPLC. You may also use norvaline or sarcosine as internal Standards. derivatization of amino acid with chiral Marfey's reagent.
The amount of ethanol in beverages has been quantified by density and infrared spectroscopy methods. The density method allows only the amount of ethanol to be quantified, while the infrared spectroscopy method allows the quantification of ethanol and, if any, the presence of contaminants such as methanol. The amount of ethanol quantified agrees to bottle declaration particularly for the infrared spectroscopy method. The infrared spectroscopy method coupled with a mathematical treatment (PCA and PLS-2) thus distinguishes itself as a fast and reliable technique for determining the amount of ethanol in beverages.
Nethra P V; L-2-aminobutyric acid is a good choice for an internal amino acid analysis standard using AMQ as a derivitization reagent. I have worked extensively using this reagent and the IS for pre-column HPLC-FLR systems for amino acid profiling. You can reach out to me for any support needed on the method. Article Assessment of the Effects of Genotype, Location, and Plantin...
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* Reading material - you may wish to get and read PMID: 14004736
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