2 isolates with the same RAPD profile and genotype but different plasmid profile?

08 August 2016 1 6K Report

Hi,

I am analysing some data coming from genotyping I am performing on carbapenem- resistant isolates of K.pneumoniae. The resistance genes should be carried on plasmids and I looked for the presence of those genes by PCR. I am analysing the data coming from plasmid profile, RAPD-PCR profile and PCR analysis on resistance genes. I notice that I have 2 isolates that have the same RAPD pattern and also the same genotype (at least for the resistance genes I looked for by PCR) , but different plasmid profiles. What could it mean? That ,maybe, some recombination/transposition event happened in the plasmid, thus modifying it in someway?

Thanks

Brian Thomas Foley

Plasmids can be gained and lost very rapidly. They can also recombine or phages and transposons can move between them, etc. Some plasmids can be stable, others are very unstable.

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