Can someone help me understand these results?

21 March 2025 1 6K Report

I did ribodepletion and then used Bioanalzyer RNA 6000 pico assay to test for any remaining rRNA. I see very sharp peak at around 100bp but i don't understand if i should be seeing a sharp peak in place of a more broader peak. Any insight is appreciated.

Michael J. Benedik

If the band is around 100nt then perhaps it is tRNA. But rRNA should mostly be larger except for the 5s/6s band, which is around that size. Would your methods have removed the larger rRNA and left the 5s?

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