Natural Product Pharmacology

Distinct natural compounds, usually plant-derived secondary metabolites that target pharmacologically relevant pathways. | Contact experts in Natural Product Pharmacology to get answers

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Questions related to Natural Product Pharmacology

Is curcumin a good candidate for wound healing besides its cytotoxic effect on Keratinocytes and fibroblasts?

Many studies showed high dose of curcumin induces ROS formation and caspase medicated apoptosis in both fibroblasts and keratinocytes which should in theory make curcumin unfit for the treatment...

30 December 2015 7,392 4 View

Is there any Cytotoxicity assay that has been reported for the novel polyketides product generated by engineering plant Polyketide Synthase Type III?

I am engineering polyketide synthase III to generate unnatural (novel) Polyketides products. I would like to test its cytotoxicity. Is there any PKS-III novel product use for cytotoxicity assay...

26 December 2015 9,069 2 View

How can I make a stock solution of 64mg/ml of reserpine essential oil?

I have to calculate the MIC of reserpine against a bacteria.

16 December 2015 8,759 3 View

Is curcumin a good candidate for wound healing besides its cytotoxic effect on Keratinocytes and fibroblasts?

Many studies showed high dose of curcumin induces ROS formation and caspase medicated apoptosis in both fibroblasts and keratinocytes which should in theory make curcumin unfit for the treatment...

12 December 2015 7,678 7 View

How should we go about statistically analyzing prepared herbal medicine samples provided they are prepared by the same method?

Herbal medicines are prepared by a standard method in Ayurveda and its efficacy is tested by animal experiments. Sometimes animal experiments are excluded and the herbal medicine is prepared in...

12 December 2015 3,151 9 View

Is there any Cytotoxicity assay that has been reported for the novel polyketides product generated by engineering plant Polyketide Synthase Type III?

I am engineering polyketide synthase III to generate unnatural (novel) Polyketides products. I would like to test its cytotoxicity. Is there any PKS-III novel product use for cytotoxicity assay...

12 December 2015 9,949 2 View

Can anybody help shed more light on growth kinetic study of endophytes isolated from roots of medicinal plants?

How do i determine cytotoxic effect of an extracted compound against human cancer lines?

12 December 2015 1,805 1 View

a a a a a a a a a a a ?

Thank you very much.

12 December 2015 4,911 1 View

Can "green tea" reduce the level of Prostaglandin D2 in our body? what is the mechanism??

"Prostaglandin D2" is considered to be harmful, hence need to lower it down in our body... any other method to lower the "Prostaglandin D2" ???

12 December 2015 9,492 2 View

How can I make a stock solution of 64mg/ml of reserpine essential oil?

I have to calculate the MIC of reserpine against a bacteria.

12 December 2015 7,197 3 View

If curcuminoid or curcumin is oxidized, does it produce benzo-quinone?

As far as I know, hydro-quinones and benzo-quinones have at least two hydroxyl groups and oxygens, respectively, bonded to one phenolic ring. But the structure of curcumin or curcuminoid have...

26 November 2015 3,712 3 View

What is the standard zone of inhibition for determining antibiotic sensitivity test using herbal or medicinal plants against microorganisms?

Is there any guideline or authentic sources as like as NCCLS for different antibiotics?

25 November 2015 3,138 3 View

Any suggestions about herbs and their effects at different inclusion level in fish?

i have conducted an experiment aimed at investigating the effect of aloe on sex ratio in tilapia. I found out that aloe at low inclusion level presented a significant difference compared to the...

11 November 2015 8,166 3 View

Has anyone done any research or trials on the cleaning efficacy of essential oils on surfaces?

Am looking at the efficacy of oils such as thieves, cloves, tea tree etc on bacteria and fungi.

11 November 2015 2,243 5 View

If curcuminoid or curcumin is oxidized, does it produce benzo-quinone?

As far as I know, hydro-quinones and benzo-quinones have at least two hydroxyl groups and oxygens, respectively, bonded to one phenolic ring. But the structure of curcumin or curcuminoid have...

11 November 2015 2,418 1 View

What is the fundamental reason associated with toxicity of purified phyto-constituents?

When we go down the lane of herbal drug extraction, we undergo the process of bioactivity guided fractionation, which further paves the way for purification of the desired herbal phytoconstituent....

11 November 2015 4,058 13 View

Any suggestions about herbs and their effects at different inclusion level in fish?

i have conducted an experiment aimed at investigating the effect of aloe on sex ratio in tilapia. I found out that aloe at low inclusion level presented a significant difference compared to the...

03 November 2015 4,491 3 View

My MTT reading is fluctuating.. can someone help me?

i have performed MTT as per Mosmann T on HepG2 cell line using different concentrations of plant extracts (1-90 ug/ml). The absorbance of all the treated groups are reduced to about half as...

01 November 2015 4,796 9 View

Is there anyone who found efficacy of botanical extracts (i.e. Parthenium, Neem etc) against natural enemies of Diamondback moth, Plutella xylostella?

Anyone who found efficacy of botanical extracts (i.e. Parthenium, Neem etc) against natural enemies of Diamondback moth, Plutella xylostella? Kindly add research papers in attachment.....Thanks

15 October 2015 5,561 4 View

Can anybody suggest the cell lines to be considered to study insulinomimetic activity of a plant's crude extract ?

I want to see the insulin secreting action of the crude plants extract over cell line as well as consumption of glucose by the cell lines.

13 October 2015 1,902 1 View

Should a plant extract be dissolved in DMF or DMSO only for antimicrobial assay?

the extract dissolved in ethyl alcohol gave negative results...could it be due to poor diffusion to agar?

11 October 2015 1,721 9 View

How effective is brine shrimp lethality assay?

I extracted fungal extract (extra cellular ) with ethyl acetate . I want to do some bioassays for this.Now I want to check cytotoxic effect of this extract. which assay will be effective for crude...

10 October 2015 6,955 8 View

Is there anyone who found efficacy of botanical extracts (i.e. Parthenium, Neem etc) against natural enemies of Diamondback moth, Plutella xylostella?

Anyone who found efficacy of botanical extracts (i.e. Parthenium, Neem etc) against natural enemies of Diamondback moth, Plutella xylostella? Kindly add research papers in attachment.....Thanks

10 October 2015 1,060 5 View

How can I quantify salicylic acid from plant leaves using spectrophotometry?

I am trying to establish the effect of priming (using a fungus) to a plant.I want to determine the level of and changes in the amount of salicylic acid as time progresses. I was not allowed to use...

10 October 2015 4,943 3 View

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