i have conducted an experiment aimed at investigating the effect of aloe on sex ratio in tilapia. I found out that aloe at low inclusion level presented a significant difference compared to the control, the following inclusion levels did not present any significant difference when compared to aloe unsupplemented fish, however, significant different was again present at the highest dosage. How do i explain this pattern of data in this field. literatures are also welcome.
This pattern has been observed in several occasions, when we were checking the effect of toxic substances on reproductive parameters and performances. It come to my mind that there is a hypothesis called Intermediate disturbance hypothesis in ecology. Cannot you check hypothesis like this.
I can help you with a few articles written by my colleagues. I hope you'll be helpful, even if they have addressed other issues. The idea is that they used various vegetable additives, and followed the growth dynamics of fish, respectivelly the disease control. Best regards!
Yes, additives can have different effects at different inclusion levels. I think it might be related to receptor saturation (when talking about organ response) and effects on intestinal microbiota (when used as a feed additive for improving growth)
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