Is there any Cytotoxicity assay that has been reported for the novel polyketides product generated by engineering plant Polyketide Synthase Type III?

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Can we use MTT-assay for high-throughput screening (HTS) for determining the cytotoxicity of drugs?

I am curious whether MTT can be use for HTS or not? i need to determine the cytotoxicity of 50 different small compounds against various panel of cancer cell line (7 cancer cell line). Is it...

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Is there any software to calculate the active site cavity of a protein?

My protein doesn't have complex structure . So, right now i am doing homo-logy modelling with other protein (with 74% sequence identity). Does, this methods give us the idea to know the active...

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Problem on Expression of arabidopsis thaliana 4-coumaroyl--coa ligase 3 (At4CL3) in E.coli??

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Is using Thermos Scientific Phusion Flash High-Fidelity PCR master Mix as my DNA polymerase applicable for DNMT1 site-directed Mutagenesis?

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Geotechnical Engineering (Proceedings of the ICE) time review?

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How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

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The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

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Why does my protein refolded to beta sheet during thermal denaturation analysis?

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Are air moisture harvesting technologies effective in combating desertification?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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State of art in natural disasters?

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Leonardo Bittencourt

Dear Adhikari

You can use the SRB, MTT assay. Both assays are accurate and not expensive.

Another alternative is perform flow cytometry labeling the cells with PI (extremelly accurate and elegant assay).

Kamal Adhikari

Dear Leonardo,

Thanks much for your information.

I am looking for some Polyketides III products that has cytotoxicity or any other biological function.