I am engineering polyketide synthase III to generate unnatural (novel) Polyketides products. I would like to test its cytotoxicity. Is there any PKS-III novel product use for cytotoxicity assay (or any other bio-assay)?
Dear Adhikari
You can use the SRB, MTT assay. Both assays are accurate and not expensive.
Another alternative is perform flow cytometry labeling the cells with PI (extremelly accurate and elegant assay).
Dear Leonardo,
Thanks much for your information.
I am looking for some Polyketides III products that has cytotoxicity or any other biological function.
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