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I've been doing cloning for a longer period. I did endless insert+vector ligation reactions but there were no clones. I found one recurring problem that I am not getting colonies in my vector only...
03 June 2025 8,450 7 View
Hello, i screened some clones in phage display strategy after 4 rounds of biopanning, which they were positive in both monoclonal and competitive phage ELISA, but after transforming into the...
25 May 2025 7,438 2 View
I'm trying to create a PSF for a small part of the human vault protein, and I am running into issues doing so. VMD seems to not be recognizing the residue names in the pdb. I noticed that the...
21 May 2025 4,000 1 View
Could you tell me what the technical meanings of "LALA-PG" and "D665" in the name of Anti-Mouse CD28 (LALA-PG) Antibody (D665) (Cat.NO.: HY-P99123A) are? For example, does it involve antibody...
19 May 2025 3,917 1 View
I am performing malondialdehyde (MDA) measurements in Leydig cells based on the method of Heath and Packer (1968), with modifications adapted to our laboratory conditions. Could you suggest any...
15 May 2025 7,087 0 View
I need to know the step by step procedure.
06 May 2025 3,419 1 View
Hello everyone, I'm currently exploring the functionalization of carbon nanotubes (CNTs) with non-covalent methods. Could you kindly recommend the most effective techniques for non-covalent,...
02 May 2025 3,632 1 View
since htert rpe-1 are adherent cells , the only additional step I do is trypsinize pellet with dmem and then proceed with trizol lysis. Is this the step that's giving me problems in rna isolation?...
29 April 2025 1,507 1 View
I have a powder that is carbon from wheat waste. What are the methods to check and know the size and characteristics of the nanoparticles of the carbon molecules? I want known methods that I can...
28 April 2025 6,409 5 View
I am calculating the encapsulation efficiency of drug loaded polymeric nanoparticles. These nanoparticles are soluble in acetone. So, acetone is used as the solvent for measuring the UV-absorbance...
26 April 2025 4,246 2 View
How effective is the application of modern sports management principles in improving administrative and financial performance in Algerian sports clubs
25 April 2025 6,030 0 View
We have to try casting high tin content aluminium -tin alloy in simple static mold casting to save cost. But it was very often having gotten parts with cracks. Can anyone summarise the current...
25 April 2025 9,243 6 View
Dear colleagues, Given that there are various tools and educational links available for learning RNA-seq data analysis, which tools are the best and have the highest processing speed? Do you...
24 April 2025 3,874 5 View
Regarding the publication: Management of first-time patellar dislocation: The ESSKA 2024 formal consensus-Part 1 DOI: 10.1002/ksa.12620 This was a collaboration within ESSKA Patellofemoral Study...
21 April 2025 2,289 1 View
In the literature, most theoretical formulas for calculating the depletion region thickness, capacitance, built-in voltage, and doping concentrations are derived for standard pn diodes. Can these...
16 April 2025 7,702 0 View
Hi, I am new to flowjo and am having a lot of trouble with figuring out the software. I have an experimental settup where each cell in my samples have a background mCherry signal and in addition...
15 April 2025 2,536 3 View
How to fix Gamma S and Gamma L in Smith chart, whether it depends on calculated reflection coefficients (gamma S& gamma L) or by optimization to get enhanced gain. As per formula method i got...
14 April 2025 4,745 6 View
Tax reform in Brazil is a reality after many decades. However, the impacts on industries are still a little explored subject, but it will change the management of these companies and...
12 April 2025 5,546 0 View
Hello, I am wanting to culture HDFs, discard the cells, and do an SDS-PAGE on the remaining ECM. Looking for a detailed protocol to do this. Thank you!
10 April 2025 5,763 2 View
I would like to export the raw spectra from the Omnian software using a Panalytical XRF, but I could not find out how to do this. I was only able to copy the graphs or export the analysis results....
10 April 2025 4,038 0 View
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03 April 2025 9,438 2 View
Please let me know how to compute RCS in HFSS. Design steps or any video guide or procedure with formula. Thanks.
02 April 2025 1,679 2 View
I have recently started working on quantifying the virulence factor of Pseudomonas aeruginosa. In winters when there was a reducing environment, I was getting a greenish tint in my bacteria. Now...
01 April 2025 763 0 View
I performed XRD analysis on a functionalized solid core. After doping it with a metal, I observed a leftward shift in one of the peaks of the functionalized solid core. What could be the reason...
31 March 2025 433 3 View