Fees and Charges

Fees and Charges are amounts charged to the patient as payer for health care services.

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Questions related to Fees and Charges

Integral Membrane Protein Purification Help?

Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...

31 May 2024 7,644 3 View

Is Casson fluid a Newtonian or non-Newtonian fluid?

30 May 2024 7,849 7 View

I want to find a formula to calculate the critical temp of superconductor?

After finding a formula to calculate the critical temp of superconductor, we can learn to elements and structure.

29 May 2024 1,398 5 View

Why do you translate my name?

My name is Mary Elizabeth C. Bernardes -de-Oliveira or M.E.C. Bernardes-de-Oliveira. But, Maria there isn't! Please correct this for me.

28 May 2024 8,291 2 View

How to calculate Leave-One-Out cross-validation coefficient q2 for a QSAR model using scikit-learn SVR?

I have a training set consisting of 39 compounds. Here is a short code to calculate LOO q2 to SVR: from sklearn.model_selection import GridSearchCV from sklearn.metrics import make_scorer,...

25 May 2024 9,017 1 View

What concentrations should be selected for hemolytic assay for antimicrobials?

I am performing hemolysis assay for antimicrobials but there's difficulty in selecting the concentrations of the antimicrobials. I have read the papers and MHC 90 was determined. Should I consider...

22 May 2024 8,944 3 View

Lots of impurities after Ni_NTA purification of his-tagged protein?

I am trying to purify 50 kDa recombinant protein with his6-tag on the c-terminal by using Ni-NTA chromatography. I am using 1 mL HisTrap columns, and I loaded my sample (E. coli lysate containing...

22 May 2024 4,605 5 View

What is the difference between to alloys named C7025 vs. C194 ?

I need to get the Technical Data Sheet of these 2 type of alloys .

21 May 2024 4,630 1 View

Unusual space group setting for phase?

When refining with HighScore Plus, inserting the CIF file for gamma-Ca2SiO4 and then refining it will show "unusual space group setting for phase: gamma-Ca2SiO4". This occurs for all gamma-Ca2SiO4...

20 May 2024 10,113 1 View

Is there any software that can take atomic positions as input (e.g. VASP POSCAR file) and give me the output of Wyckoff positions?

Is there any software that can take atomic positions as input (e.g. VASP POSCAR file) and give me the output of Wyckoff positions? I am trying to find vacancies of some specific Wyckoff positions...

20 May 2024 7,904 3 View

Why multiple changes in UNDER REVIEW status date?

One of my papers was submitted in Springer journal on 16th July 2023. After two months later, the status changed to "UNDER REVIEW" on 19th Sept. 2023. The status changed to "UNDER REVIEW" twice...

19 May 2024 5,016 0 View

Best method for extracting RNA from low biomass of Ectocarpus (filamentous brown algae)?

Hello everyone! I am currently working with the model organism Ectocarpus (filamentous brown algae). Our team is trying to standardize a protocol for RNA extraction using low biomass (around 10...

19 May 2024 3,960 1 View

How does social media (facebook) influence a women’s ‘traditional’ role within relationships particularly in terms of parenthood?

any ideas on how to refine this? or answers i beg

19 May 2024 9,693 2 View

How to calculate the c lattice parameter of MXene?

I need to find the c lattice parameter of HF etched Ti3C2 MXene and delaminated Ti3C2 MXene. Papers are reporting an increase in c lattice parameter after delamination. Any formulas, calculations...

18 May 2024 8,493 0 View

96 well filter plate recycle protocol?

I am using 96 well filter plates(0.45um, hydrophilic) for protein particle removal, but they are very expensive and I would like to ask if anyone has tried reusing them. I am recycling the amicon...

16 May 2024 6,055 0 View

Kindly suggest best journals for biodiversity having fast publication process?

For new record of mushroom

15 May 2024 5,877 0 View

I want to publish a paper on reaserch how can i do that ?

I want to publish a paper on ResearchGate how can I do that?

15 May 2024 1,517 2 View

How to get "density" and "viscosity" for cavitation(water vapor) for cavitating Francis turbine?

Hello there I am simulating cavitation for a Francis turbine. Francis turbine is rotating with 826 rpm. I have two questions; 1- Vapor pressure for my case is 2100 Pascal, and Temperature is 25...

14 May 2024 4,448 7 View

What keratinocyte cell lines or primary cells do you utilize for wound healing assays?

Hello, I am currently working on the design and fabrication of scaffolds for wound healing. For in vitro tests, which cells do you use? Fibroblasts, keratinocytes? Primary ones or cell lines?

14 May 2024 6,830 2 View

What is the rationale for both C- Myc tag and 6xHis tag in same plasmid. Can i use only his tag for mammalian expression?

I wanted to express protein in mammalian cells for purification I need 6XHis tag, however, plasmid synthesis company have vector with Myc and his tag. So do I need both or only his tag.

13 May 2024 1,001 4 View

Sample with 8M urea causes high pressure during IMAC?

Hi all, I have a problem with the high pressure of my HisPrep column. So these are the steps did before I loaded the sample: 1. Frozen cell pellet was resolubilized directly with lysis buffer...

13 May 2024 1,282 2 View

Why is Services, value added (% of GDP) not equal to the Services value-added/GDP in the World Bank data?

The World Bank database has a bug! The share of value added of each economic sector to GDP (%) must be equal to the value added divided by GDP in a certain year and country. However, they are not...

11 May 2024 652 1 View

Why now can't ResearchGate send my published article ("too big") to people requesting it?

https://www.researchgate.net/profile/Peter-Messer-2/research My article entitled "A Review of Nearctic Selenophorus ..." now can't be sent to people requesting via ResearchGate. Error message:...

11 May 2024 8,435 15 View

Protein degradation? how to concentrate low MW proteins?

Hi everyone! So I am trying to concentrate my recombinant proteins in order to make some assays with them, but I need a "considerable" concentration of each. One of my proteins is around 9kDa and...

09 May 2024 5,548 2 View