96 well filter plate recycle protocol?

16 May 2024 0 6K Report

I am using 96 well filter plates(0.45um, hydrophilic) for protein particle removal, but they are very expensive and I would like to ask if anyone has tried reusing them.

I am recycling the amicon tube 2-3 times by washing it with ethanol and distilled water,

I haven't tried reusing the filter plate, and it takes a week to prepare a sample for testing, so I would like to ask if anyone has tried reusing it.

If you have, I would also like to know how you wash them.

More Nakyeong Koo's questions See All

Is it possible to remove trapezoidal shape artifact?

Dear all, I executed oscillatory TES with trapezoidal shape during EEG recording. Although I have some stimulation free intervals that I can analyse the signals, it would be good to see what's...

19 May 2016 1,537 3 View

What would be the best way to reduce the public's fear of radioactivity ("radiophobia')?

After the three big nuclear accidents (TMI, Chernobyl, Fukushima), radiophobia has become one of the most difficult obstacles to using nuclear energy. While many attempts have been made to resolve...

01 January 1970 7,025 23 View

What would be the future of nuclear industry?

It is typically said that an industry life consists of four cycles -- startup, growth, maturity, and renewal or decline. I think the nuclear industry is in the stage of renewal or decline. And...

01 January 1970 5,025 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View