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I am working on Spinal muscular atrophy (SMA). My target is to develop an Antisense Oligonucleotide (ASO) based drug for the treatment of this disease. For this, I want to see if the ASO I am...
17 July 2023 5,354 1 View
I'm looking for videos (or eventually photos) of duchenne and non-duchenne smiles that I can use in my research for my thesis about influence of social exclusion on recognition of real and fake...
17 July 2023 1,966 1 View
Hi, I am looking for daily or monthly data LAI data set for calibrating with monthly simulated data. I do have MODIS 8-day data which aggregated to daily data using interpolation method, but the...
17 July 2023 4,328 2 View
Is a woman need a full hijab from thermally isolated clothes in summer?
17 July 2023 673 1 View
We want to know if it is possible to have a PMMA layer on U-PVC profiles by co extruding PMMA on U-PVC profile while the process of manufacturing profiles. Is it necessary to mix PMMA with...
16 July 2023 8,660 0 View
In synchronous SRAM design, along with SRAM array there is address register and data register. Is these registers are also used for storing the data or they are just for delay?
16 July 2023 2,367 0 View
Why does ellipsometry aim to build a model of a sample that reproduces experimental data ($\psi$ and $\Delta$, which are often defined by the total reflection coefficients:...
15 July 2023 8,286 3 View
What are the roles of microorganisms in wastewater recycling and role of microorganism in the recycling of elements?
15 July 2023 3,752 3 View
Since I am working with anaerobes I need to know the standard sterilization procedure to be followed within the chamber. Thank you
14 July 2023 6,612 2 View
I have two types of template (RNA) derived from two different types of tissue (normal and tumor). Which one I should add to well with endogenous control primers/probe (GAPDH) at qPCR plate? I know...
14 July 2023 124 2 View
4 protons peak may be CH2 near a heteroatom .
14 July 2023 1,056 1 View
Does drying leaves and stems in a high humidity environment influence the bioactive molecules like flavonoids and polyphenols or no?
13 July 2023 5,150 5 View
For sanger sequencing reaction with BigDye terminator mix, I will need to purify the excess unincorporated terminators, primers, dNTPs from my PCR product. But in times when I won't be able to...
13 July 2023 308 3 View
I've been trying to induce an inflammatory response using LPS (Sigma O127:B8 and O55:B5) using the immortalized human myometrial cells (TERT-HM) in our lab but have failed to get a significant IL6...
13 July 2023 1,666 3 View
I need to load a drug on polymer nanoparticle, most of procedures used triethylamine TEM with chloroform for solubilization the drug, but I have trimethyl amine TMA instead of TEM, my questions is...
13 July 2023 2,762 2 View
We need to collect a fair amount of Erpobdella octoculata leeches for running ecotox experiments, but struggling to find them in abundance in Sheffield location. Anyone got any tips for sampling...
12 July 2023 2,571 3 View
Exism movements can not be expected to coexist peacefully with the democratic institutions checking the working of the democratic environment under which they come to exist, when in power or when...
12 July 2023 656 0 View
As forests are large ecosystems, their inventories require use of representative sample. Then, what is the minimum percentage of forest area to be sampled to minimise the errors and get acceptable...
12 July 2023 8,615 3 View
The idea is that I want to perform continuous hydrological modeling while integrating the Curve Number (CN). Thank you.
12 July 2023 6,379 7 View
I'm planning to conduct Metagenomic analyses on DNA extracted from soil using the ITS and 16S primers. However, I'm getting bands at 2k or 3k on the gel, even though my ratios and DNA...
12 July 2023 8,695 3 View
I am writing to inquire about the low assignment ratio (19%) that I obtained using FeatureCounts in my RNA-seq analysis. I would like to confirm whether this is a normal result, and if possible,...
12 July 2023 8,626 2 View
Is there any technique, method, or instrument available to simplify the counting of orchid seeds, which is currently a challenging task due to their minute spore like size and large quantity of...
12 July 2023 6,914 3 View
I am trying assemble transcriptome data from a Tubificinae annelid, genus Limnodrilus. I sent in tissue and eukaryotic RNA seq was run successfully (poly-A tail RNAs). However, the genomes I...
11 July 2023 3,820 3 View
Logistic Regression type
11 July 2023 937 3 View