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Hello colleagues! I am currently conducting research on the effect of dopants and radiation on ZnO and similar metal oxide thin films. In order to publish results that are as close as possible to...
23 May 2025 9,931 8 View
I performed a Histrap IMAC purification to purify a His-tagged archaeal TF after an overexpression protocol. I did this experiment two times for the same TF. I only slightly altered the...
22 May 2025 4,115 4 View
I am running the TLC on plant leaves extract and got the results with spots that colorless under visible light but have bluish and pinkish coloration under UV 366 nm. Then, I sprayed it with AlCl3...
21 May 2025 2,089 3 View
I am doing flow cytometry for cell cycle with E. coli after induction of proteins that cause cell death. The procedure I followed consists in growing cells to an OD of around 0.2 take a pre...
20 May 2025 5,870 1 View
how to remove this paper now as it was not his paper we removed it from our profile
20 May 2025 9,220 0 View
I would like to perform immunofluorescence (IF) on testicular biopsy samples using frozen sections. Should I fix the tissues prior to sectioning, or do you recommend fixation after sectioning?...
19 May 2025 3,301 1 View
I tried to run malmq2 code for an unbalanced panel data in Stata 17. But an error message is generated like shown below. (existing lmalmq2.mlib compiled with Stata ???; need to recompile for...
15 May 2025 1,540 0 View
Dear ResearchGate and fellow Members, I cannot guess why RG management decided to abandon the members comments section that IMHO serves as a useful feedback for authors but if the reason(s) was...
15 May 2025 5,143 7 View
Gentle Researchers, I am measuring thermal diffusivity of distilled water through LFA 447 nanoflash, but the value I am receiving is very high 0.22 mm2/s. The 2nd layer of the sample holder have...
13 May 2025 5,615 0 View
I am retired from Columbia University and no longer use that email. How can I get verified?
13 May 2025 664 0 View
Can the same model be used for nonlinear analysis like in linear analysis? No, you will have to add a ton of information into the computer model to do nonlinear analysis. You will have to add...
11 May 2025 560 1 View
I would like to study a protein-protein interaction between a cytosolic protein and a membrane protein. Can someone suggest an effective method to pull down the membrane protein?
04 May 2025 3,511 0 View
We believe the correct parsec is 3.085122.... times 10^16 m. This should be the most accurate value of the parsec, it is not a function of Pi but a function of the golden ratio and a new...
01 May 2025 4,124 9 View
How do I isolate plasma cells from blood? What’s the best method for pulling out B-cells (especially antibody-secreting cells) from blood samples?
30 April 2025 8,294 4 View
I am developing a stick sunscreen with an anhydrous base structured by waxes and vegetable oils. During rheological characterization (viscosity × temperature), I observed that the formulation...
29 April 2025 3,866 0 View
My question is the same as the title. I have an electronic multichannel pipette and was wondering if it's an effective and reliable method of loading a 96-well ELISA plate or if I should stick to...
25 April 2025 5,316 1 View
Exploring Open Source Information for Cyber Threat Intelligence. This is our research topic. Can some one have any expertise on it?
24 April 2025 6,277 0 View
Hi everyone, I’ve recently experienced contamination in my podocyte cell culture, and my colleague suspects it might be yeast. I’m trying to understand what could have caused this contamination....
23 April 2025 801 4 View
When I measure the fluorescent emission intensity, there appears two peaks very close and with similar peak hight. I checked with different solvents, but it still the same. Is it ok to continue...
23 April 2025 1,754 1 View
"En las ciencias del ambiente construido, cada escuela de pensamiento, departamento o facultad ha interpretado el hábitat desde su posición sin justificar su interpretación en relación con el...
23 April 2025 154 4 View
Does anyone have experience or suggestions on how to dissolve niraparib for in vivo injections in mice? Any suggestions or protocols would be greatly appreciated
23 April 2025 4,090 2 View
Hello everyone, I’m working on isolating oligosaccharide compounds from the crude ethanol extract of medicinal plants. I plan to use column chromatography for the isolation and would like to...
22 April 2025 10,025 6 View
deeply understand dermatomyositis and SLE ?
20 April 2025 5,679 1 View
I now want to use matlab code to optimize the chemical kinetics model. I don't know how to use matlab to call chemkin software. I hope you can help me.
18 April 2025 4,969 0 View