My question is the same as the title. I have an electronic multichannel pipette and was wondering if it's an effective and reliable method of loading a 96-well ELISA plate or if I should stick to using a manual pipette? If it has a repeater function can I also use that i.e. if the protocol requires 100ul/well of sample and I'm running triplicates, can I aspirate 300ul of sample and then dispense three times continuously? Would that increase or decrease my CVs?
Of course! Justin!
Electronic pipettes are very suitable for batch loading and repeated well operations of 96-well plates! It is a good tool that can not only significantly improve efficiency, but also theoretically have a smaller CV value than manual loading.
If the sample has high viscosity, surface tension, or is prone to bubbles, it may affect the accuracy of the pipette loading.
Because it is not clear what the object you are loading is, it is recommended to conduct a preliminary experiment before the formal experiment.
Use the same sample and loading method to load on multiple 96-well plates, and then measure the absorbance or other indicators of each well to determine whether the CV value meets your expectations.
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