I would like to study a protein-protein interaction between a cytosolic protein and a membrane protein. Can someone suggest an effective method to pull down the membrane protein?
Any specific technique, please suggest to me?
04 October 2023 8,796 4 View
Looking for the questionnaire on EMOTIONAL STABILITY SCALE developed by CHATURVEDI (2010) with description.
21 May 2022 621 1 View
I need to acid digest the soil sample and vegetative for heavy metal analysis and want to make ready the sample for analysis. But my question is, what is the stability of the acid digester sample...
09 January 2022 2,027 8 View
Dear Sir/Madam, Instead of below mentioned article some other article is uploaded in your website please check Chinese Herbal Medicine for the Optimal Management of Polycystic Ovary...
15 July 2021 4,189 1 View
This is to understand how the concepts of statistical mechanics is applied in astrophysics.
01 April 2021 9,141 4 View
It would be interesting to know, how the scientific community is contributing to the COVID-19 crisis. How chemical/mechanical/material/metallurgy concepts are being used to produce anti-COVID-19...
16 May 2020 5,313 3 View
Self-actualization It refer to the realization of a person's potential, self-fulfillment, seeking personal growth and peak experiences. Maslow (1943) describes this level as the desire to...
09 April 2020 9,423 4 View
I have a list of compounds, these are well-researched bioactive compounds. I want to know the available databases that I can see to compute the toxicity.
11 March 2020 7,340 3 View
I need an example of a detailed reporting of pathdiagram.Pls help
10 January 2020 8,808 3 View
Behavioural Sciences Research in behavioural science like anthropology, sociology and psychology has provided the subject matter for human resource development. Behavioural science era led to the...
24 December 2019 4,152 5 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,294 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View