When I measure the fluorescent emission intensity, there appears two peaks very close and with similar peak hight. I checked with different solvents, but it still the same. Is it ok to continue like this?
Adam B Shapiro
Take an emission spectrum of the solvent alone to see whether one of the peaks is coming from Raman scattering by the solvent. If so, you can subtract the solvent spectrum from the analyte spectrum.
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