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I am encountering issues while designing SSR primers using Primer3. The provided script for the p3_in.pl and p3_out.pl files is not functioning correctly. Specifically, the GC% for each primer is...
28 May 2025 7,188 0 View
Esta pregunta puede servir como punto de partida para profundizar en cómo la inteligencia artificial está transformando la educación y cómo se puede utilizar de manera ética y efectiva
28 May 2025 593 4 View
On [email protected] I received this email: ------------------- 📷Jean Philippe Blankert invited you to join their network on ResearchGate and confirm authorship of your publications. 📷 Ai...
27 May 2025 612 2 View
Researchers can effectively collaborate with policymakers by engaging in ongoing communication, providing actionable and relevant research findings, and building trust and credibility through...
27 May 2025 956 5 View
Si sabemos que la contaminación ambiental nos perjudica a todos y daña el planeta de forma irreversible, ¿por qué la sociedad sigue priorizando el crecimiento económico y el consumo excesivo sobre...
27 May 2025 6,602 7 View
I've been suggested this as an alternative to EtBr, it's also not expensive, so I would like to know if anybody has used it and how it is.
23 May 2025 4,577 1 View
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21 May 2025 3,536 4 View
Dear all, Someone with a similar name has falsely claimed authorship on several of my publications. ResearchGate replies that they were “unable to confirm” me as the author and tells me to...
19 May 2025 8,799 2 View
The question focuses on finding out the opinions of public relations experts on the gaps that may exist between the public relations department and senior management.
17 May 2025 10,060 4 View
For the ChIRP MS experiment, I am sonicating the crosslinked HT29 cell lysate using diagenode bioruptor. The cell lysis buffer has 50mM tris-Cl (pH7.0), 10mM EDTA (pH8.0), 1% SDS, 1mM PMSF, 1X...
15 May 2025 9,958 0 View
Dear science community, I added a substance to samples with 2% increment (0 (original), 2, 4, 6, 8, 10%). A regression model combined with measured variables was employed to predict the level of...
09 May 2025 2,568 1 View
A favorable magmatic signature for a porphyry copper may not indicate tonnage of copper...but there might be a lower bound. Either in copper tonnage, or volume of source rock. Fertility is not...
09 May 2025 6,813 2 View
I recently added my third publication to my ResearchGate profile. While the total publication count correctly shows '3', the new publication is not listed in my publications display when viewed...
09 May 2025 9,531 2 View
The h-index and i10-index provided by Google Scholar are generally more robust and dynamic compared to those on ResearchGate, which can be less transparent and less frequently updated. Given this,...
07 May 2025 2,696 2 View
How can machine learning detect financial fraud in real-time?
07 May 2025 3,053 6 View
I have purified a recombinant protein with a His tag(no cleavage site ) for oral administration in mice (via orogastrically) . Is it necessary to remove the His-tag before invivo studies?? If so ,...
03 May 2025 8,241 5 View
How AI impacts on functional literacy?
03 May 2025 1,996 4 View
This question explores whether the use of complex medical terminology in insurance policies is intentional to create advantages for insurance companies. By examining this, one can consider whether...
02 May 2025 6,384 0 View
There is some uncertainty regarding the energy calibration in our UPS measurements. We used gold (Au), a metallic reference, alongside our samples for instrumental calibration. However, since our...
30 April 2025 5,870 2 View
Dear all, I am finding that some of the spleen sections that I make at the cryostat and attach to my glass slides, regardless of the tissue NOT demonstrating any cuts during the slicing, show...
29 April 2025 8,819 1 View
Why must nuclear reactant be safeguarded? What impact would nuclear exposure caused to humanity
26 April 2025 419 3 View
We are currently working on purification of human recombinant Factor IX (rhFIX) using three independent chromatographic steps. We experience significant loss of activity during purification and...
23 April 2025 7,336 1 View
How can we differentiate between conceptual framework, empirical framework and Theoretical framework in literature review in applied science research.
22 April 2025 263 0 View
Hello everyone! I've recently ordered a DuoSet™ ELISA kit from R&D Systems, and while reviewing the protocol, I came across a few points that raised some questions. If anyone has experience...
22 April 2025 3,694 2 View