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How to remove pellet from SS Dye ?

I have made pellet of Tungsten powder, before that i clean dye with IPA. Now my pellet is stuck in the dye. can any body have idea how to remove it, So that dye do not get damage. i don't care...

15 July 2025 7,672 0 View

Is the lysosomal pH indicator probe CypHer 5 a live cell dye? Can it pass through the plasma membrane?

I am doing an experiment to monitor the pH of lysosomes in cells and want to try the probe CypHer 5. But there are some questions: First, is it a live cell dye? After all, we want to track the...

15 July 2025 4,654 1 View

Why expired drugs used as corrosion inhibitors with acidic media only, and rarley used with saline solution ?

especially with the metronidazole drug

15 July 2025 2,672 2 View

How to decide how many cells to be plated in a 96-well plate? Do I need to draw a cell growth curve?

I tried to search for a proper protocol for the log phase test, but the webpages showed that all protocols are for bacterial growth curve or viral growth curve. Protocols for cell growth curve...

14 July 2025 2,098 1 View

Which method is best suited? How long before the drug should be added?

In C. elegans study, some literature take agar volume into consideration while calculating a drug dose regardless of the liquid or solid NGM. Which method is more preferable for solid NGM seeded...

12 July 2025 3,912 6 View

What is the optimum optical density of ovarian cancer cell lines for cell proliferation assay?

I am working with two cell lines and mostly I got 0.5 OD in control cells even when I increase the cell numbers and I am using Alamar blue assay for 3 hours, is that ok or I should get higher...

10 July 2025 8,151 2 View

For moderately non-normal repeated weight data with baseline differences, should I use RM ANOVA (with Greenhouse–Geisser) or a non-parametric test?

I have weight data from 4 animal groups measured at multiple time points. Some data at 2 time points is moderately non-normal. Should I still use repeated measures ANOVA with Greenhouse–Geisser...

08 July 2025 5,139 3 View

Good morning, I have accidentally indicated that I am the author of an article that I am not. How can I remove it from my authorship?

I made a mistake when I pressed the button

08 July 2025 692 1 View

If I want to monitor tumour growth in mice without performing dissection, what non-invasive techniques are typically used?

In the reply when the person asked that what is the process I said bioluminescence luciferase assay can be an alternative to the process. My colleague said no. please give me insights that do you...

07 July 2025 2,699 3 View

Guide me on Systematic review literature its urgent?

Can I write this line in my : "The proposed systematic review and meta-analysis will be conducted following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses)" since I...

07 July 2025 5,991 2 View

Why do adolescent girls spend so long in the bathroom?

Tell her she’s important to you. For a woman to feel the desire for sex, she needs to feel loved, adored and significant. (Allan & Barbara Pease)

06 July 2025 8,774 2 View

Countries are bound by relative wealth but advanced by optimism?

If a person increases his income by one order of magnitude (e.g., from $10,000 to $100,000) and another person decreases his income by one order of magnitude (e.g., from $1,000,000 to $100,000),...

05 July 2025 9,066 4 View

What could be the reasons for observing cell debris, aggregation, and fragmented cells during primary neuron culture?

Hello Professors and Senior Researchers, I am currently working on primary neuron culture. I would like to share an issue I have encountered: Despite passing the cell suspension through a filter,...

05 July 2025 8,523 2 View

Ιs the This shift from philosophical and conceptual thinking to a much more technically oriented approach good for physics?

This shift from philosophical and conceptual thinking to a much more technically oriented approach can also be observed in the generations of researchers that followed the discovery of the...

05 July 2025 4,701 1 View

Why are theories in sexology accepted as fact with so little supporting evidence?

I stumbled across the mysterious G-spot while looking at orgasm dysfunction. I noticed there were only a handful of studies on the subject, which relied on very small sample sizes. I found it...

04 July 2025 1,787 0 View

How commonly the PEEK implants are being used in Arthroplasty?

What are the pros, cons, and published outcomes of these implants? Please share your experiences.

03 July 2025 7,216 3 View

Is Heisenberg's matrix mechanism truly flawed? Can it be improved?

The answer to both questions is yes, but Schrödinger's original iron guards, made from the remainder of physicists and mathematicians, remain safe and effective. Schrödinger's iron guards in...

02 July 2025 8,108 2 View

Why my TEER is low in Caco-2 ?

I try to grow Caco-2 cells monolayer and have tested a range of cell seeding densities, from 4 × 10⁶ to 4 × 10⁴ cells/mL. I am using 12-well Transwell inserts with PET membranes (0.4 µm pore...

02 July 2025 5,092 2 View

¿Cuáles son los beneficios de el modelo de cuidados paliativos continuos?

El modelo es la construcción de una idea que se visualiza y se concreta en acciones específicas para disminuir el sufrimiento asociado al cáncer.

01 July 2025 1,566 0 View

Can anyone provide me JCPDS XRD files for Au, Pt Nanoparticles and g-C₃N₄?

Hello everyone, I’m currently analyzing XRD patterns and looking for the JCPDS (PDF) reference files for the following materials: Gold (Au) – JCPDS Card No. 04-0784 Platinum (Pt) – JCPDS Card No....

01 July 2025 1,463 1 View

Who else wants to develop & use ASI to help save us from us?

Am I the only ASI R&D guy developing ASI assistants for something other than helping mega-hucksters find better ways to sell more shoddy/extra stuff & 'services' to more suckers for more...

29 June 2025 10,021 3 View

Requesting Endorsement for arXiv Submission?

Subject: Request for arXiv endorsement in physics.gen-ph I recently completed a research paper proposing that force, gravity, and inertia arise from gradients in time flow—a unified temporal...

28 June 2025 2,089 2 View

What would be the impact of the use of AI tools in Higher Education?

1. Impact to Teaching-Learning Process; 2. Analytical and Critical Thinking Skills of the Students; 3. Problem Solving and Life Skills; and 4. Learning Retention.

25 June 2025 4,411 4 View

G and D Peaks are missing in my Raman Spectra of Metal Organic Framework (MOF) derived Carbon?

Hi Everyone, Currently, I am converting my Nickel-BDC-DABCO MOF to N-Doped Carbon material using Pyrolysis method in tube furnace. Methodology: I am conducting this experiment at 800 degrees at...

25 June 2025 5,576 0 View