In the reply when the person asked that what is the process I said bioluminescence luciferase assay can be an alternative to the process. My colleague said no. please give me insights that do you agree or not.
Hello Sayandeep Mukherjee,
Yes, I agree. For bioluminescence luciferase assay, you will have to genetically modified the tumor cells to express luciferase and make D-luciferin (substrate) available to the luciferase enzyme in the tumor cells by injecting D-luciferin in mice. Also, you need to note that for accurate measurements, you will have to minimize background signals from the animal's own tissues.
You will need a CCD camera to visualize and quantify the tumor's bioluminescence. By repeatedly imaging the mice, you will be able to track tumor growth and metastasis. The intensity of bioluminescence will correlate with the number of tumor cells, allowing for quantification of tumor burden.
Best,
Where is this tumor located? If it is somewhere on the hindpaw, you can measure the volume of water that would be displaced from the tiny beaker by this limb.
