Q-Dots or other high yield fluorophore that exite at ~254nm and emit at ~300nm?

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Comercial "stratum corneum substitute" lipid mixture?

Greetings We want to look at the effect of Stratum Corneum composition on bacterial attachment. Does anyone know a commercially available "stratum corneum substitute" lipid mixture? Or is willing...

17 November 2019 1,383 2 View

Extraction of Heme A, Heme B and Heme O?

Greetings I am looking for a trusted protocol for extraction of Heme A, Heme B and Heme O, to be used as standards in HPLC analysis. Alternatively if you know of a commercial source also...

27 May 2019 5,240 2 View

Can I connect HP1047A RI detector to Agilent 1200 HPLC system?

Greetings We need to buy an RI detector for our Agilent 1200 HPLC system (connected to a computer running ChemStation). Given the high price of new detector I thought about buying a used one....

19 October 2017 6,270 9 View

Can anyone answer my question in hydrochemistry of boreal wetlands?

I am working on time series in chemical composition of shallow ground water samples collected between 1994 and 2010 at the largest known boreal wetland. Aside from the understandable and well...

15 June 2014 2,792 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

Ofir Tal

Sounds like Tyrosines abs and emission. Although I'm not sure regarding the q.y..

Fabrizio Messina

Tyrosine QY is very low. PTP is indeed a good suggestion (you can solve it in methanol or ethanol in reasonable concentration). If 350 nm emission still works, another alternative is 2,5-Diphenyloxazole..