35 Questions 21 Answers 0 Followers
Questions related from Wei Li
4.5 is the maximum capacity of rAAV. I wanna it having about 8 kb capacity and less immunogenecity. If I use adenovirus, it may cause high immunogenecity, through it has about 8 kb capacity.
24 August 2018 8,283 2 View
e.g. lentivirus can be used in delivering of gene of interest into both dividing or non-dividing cells, while gamma-retrovirus is limited to dividing cell. In mixture population of non-dividing...
23 August 2018 4,848 2 View
I wish the books or references that could tell me why the morphology of these cells happen and what implications does such morphology will give us under designated conditions.
18 August 2018 8,805 0 View
Except Addgene plasmid 101
12 May 2018 8,097 3 View
I think it's because of internal reasons (e.g. temperature-sensitive ori) in plasmid itself and natural reasons (consume lots of resources in cells and probably digested by protease). In terms of...
12 May 2018 3,295 1 View
I wanna use Cas9/sgRNA complex to edit E. coli genome. Is it workable? Cas9/sgRNA complex is in solution. Transformation method could be chemical (Ca+) or electrophoration.
07 May 2018 5,691 7 View
I am studying the mechanism of homologous direct recombination (HDR).
05 May 2018 6,511 2 View
I wanna know why transposon only jump once in the genome of a cell? mechanism
28 April 2018 4,161 3 View
if they have, what type of harms?
22 April 2018 6,770 4 View
Are there any an obvious tendency showing this point?
31 March 2018 7,226 0 View
what sorts of problems are difficult to solve when you do HTS using thoese methods.
30 March 2018 6,164 3 View
Limitation: 1) target specific gene: 2) do epigenetic modification:
18 March 2018 3,426 3 View
Could you summarize and share your experience to me?
02 March 2018 9,981 0 View
please see this two figure. maybe drawing these two figures could require R script. Are there any references or papers talking about how to make these two figures?
01 March 2018 6,985 3 View
Could you categorize the types of diseases according to changes of gene sequence, gene modification, others.
10 February 2018 7,508 1 View
the spot in microchip is quite small, so I guess fluorescence data in 96-well plates after being detected by Fluorescence Detector also need to be corrected using some ways of data handling
04 February 2018 707 2 View
add all numbers outside of the bracket in "matches"? like 1+1+1+1+1+.....
30 January 2018 2,388 4 View
could take an example in the below picture.
30 January 2018 7,774 3 View
please, look this example, thanks
30 January 2018 9,236 5 View
How to relatively accurately quantify the SDS-PAGE gel and DNA electrophoresis gel? Any methods?
27 January 2018 7,299 3 View
e.g. I have a set of GFP-based membrane proteins, I detect the fluorescence intensity. I could say the more intense detected fluorescence, the more the amount of fusion protein. But what is the...
26 January 2018 601 4 View
Experiment: express a simple protein (e.g. membrane protein of E. coli) in E. coli in a way of fusing GFP and membrane protein. Linear? or exponential or else ??
22 January 2018 2,293 3 View
I know TEV can cut ENLYFQ G.
22 January 2018 3,521 3 View
As shown in this picture, if the rate of protein sequence coverage is a result that the number of matched amino acids among MS/MS peptides is divided by the total number of amino acids in searched...
19 January 2018 3,899 1 View
I wanna produce and publish a Cas9 gene therapy drug, but I don't know how to do, I lack of example and real case for this project. Could you recommend any real cases to me?
18 January 2018 9,108 0 View
it seems many senario that we have to/could do log transformation) e.g. make growth curve of bacteria, OD650 values could be transformed into corresponding log value, then plot growth curve. When...
02 January 2018 6,953 4 View
include ethic part, but not limited to this. mainly in operation and technology Is there any hindrance to this
23 October 2017 3,193 3 View
A tissue-specific Knockout mouse defines a conditional biological model in which a target gene can be specifically inactivated in specific tissue(s); in all other tissues, the target gene exhibits...
22 October 2017 7,310 0 View
22 October 2017 5,611 0 View
1. Physiology is altered especially in 'immortalised' cell lines. 2. Cells pick up many mutations 3. Growth in nutrient rich media, often alters response to growth factors. 4. Environment cannot...
11 October 2017 401 0 View
Could you post some examples or names of proteins here. Or tell me relevent references?
11 October 2017 1,249 1 View
Can I calculate it? if I can, how to calculate? if I lack of conditions? Or Why I cannot?
10 October 2017 3,460 2 View
A chemical question in fact.
10 October 2017 5,425 1 View
Can you list any differences of protein production by animal cell culture and microbial culture?
08 October 2017 7,757 4 View
A series of discussion about experimental details in the paper, people could post your question, I will give my ideas about your questions, or further discuss with you.
01 January 1970 8,525 0 View
