Are there any authentic papers explaining homologous direct recombination pathway in mammal cells?

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Problems about acousto-optic coupling in Comsol, how to check whether the moving mesh is correct?

I’m trying to reproduce the acousto-optic coupling simulation in the paper Frequency–angular resolving LiDAR using chip-scale acousto-optic beam steering. LNOI(lithium niobate on insulator) is...

13 August 2024 9,795 0 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,242 2 View

How to solve the problem of unconscious teeth grinding during sleep at night?

One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...

08 August 2024 1,366 5 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

How to get LP fiber modes directly from Comsol?

Hi there, I'm new to modelling with comsol. I would like to ask if it is possible for comsol to output linearly polarized (LP) modes? I tried modelling a simple single mode fiber with an enlarged...

23 July 2024 4,019 3 View

What is the future scope of acoustic emission?

17 July 2024 1,510 1 View

How to understand Instantaneous SINR (ISINR) ?

I read two papers which uses Instantaneous SINR (ISINR) as criterion for performance evaluation. In the two papers below, it seems the desired signal (numerator) in ISINR does not involve...

16 July 2024 4,545 0 View

Abnormal data in in-vitro drug release study?

I'm doing in-vitro drug release study using dialysis bag method and collecting samples from the external medium at several time points, subjecting to UV-Vis spectrophotometry. But my absorbance...

14 July 2024 6,829 2 View

Are my cells contaminated with mycoplasma?

I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...

11 July 2024 7,786 3 View

Black dots like contamination in my cell cultures. What are they?

There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...

11 July 2024 4,829 0 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Learning in Animals with Unlimited versus Limited Neurogenesis?

“Yerkes (1912) trained an earthworm to choose one arm of a T-maze, using electric shock as punishment for error and the moist burrow as reward for correct choice. The habit was acquired in twenty...

31 July 2024 8,809 0 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

How does guest authorship affect authenticity in research and what kind of authorship issues takes place in publication misconduct?

12 July 2024 2,994 0 View

How to troubleshoot error E1 on Biorad Powerpac Basic despite using sufficient and fresh buffer?

I have used this BIorad Powerpac Basic for 4 years now, before which it was used for another 5-6 years. Generally we addressed the E1 error by compensating with fresh buffer and/or filling the...

01 July 2024 7,104 1 View

Is DNase I (RNase-Free) necessary to be used before cDNA synthesis?

I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...

27 June 2024 2,951 5 View

How can repair crack in alumina to make it helium leak tight for ultra high vacuum application??

I want to close crack in alumina kindly suggest any glue or epoxy to close leak.

27 June 2024 10,110 1 View

Is it okay to grow bacteria in 30 for cloning? What is the lowest mass of Vecor I can take for cloning? what should be the optimum insert ratio?

I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...

26 June 2024 3,735 3 View

What is wrong with Site-Directed Mutagenesis?

I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...

24 June 2024 6,079 4 View