22 Questions 7 Answers 0 Followers
Questions related from Shilpi - Gupta
Hi, anyone can please tell me is crystal structure of p50-p50 dimer (P-P) available? I am able to find only p50 homodimer complexed to DNA on PDB site. I need to do docking with its 10 bp binding...
29 November 2018 1,146 2 View
Can anyone has standardized protocol for Transfection in raw cell line? I have Thermo Ltx reagent? Is it vary with plasmid type? I have Pcdna 3with flag and his tag?? please suggest plasmid conc....
15 January 2018 6,380 4 View
Hi, I need to know about cell counting for time point experiments- I am working on Raw 264.7 cells which has 11 hours doubling time. I use to seed 1 Million cells in six well plate which has...
01 January 2018 2,227 2 View
HI, all. Can anyone suggest me morphology of this cell line- I hv a dubt, is this only sperical morphology or dual with fibrocytic too? I have read that mostly it should be circular and it will...
25 December 2017 261 3 View
Dear All, is anyone has idea for international conference in 2017 on Helicobacter parthenogenesis, host immune response etc.
25 July 2017 1,026 2 View
HI, all can we able to differentiate 5kb base pair difference with Blue native Page gel electrophoresis. Anyone has idea for some otehr technique to differentiate homodimer formation for NFkb1(100...
19 January 2017 2,836 1 View
HI ALL, I am working on H.pylori mediated gastritis and looking for nfkb11 expression. I am getting upregulation of p50, I like to know possibility of homo and heterodimer formation when infecting...
19 January 2017 466 0 View
Hi Everyone, I am working on rna seq data, In my data, I have many genes which shows zero read in wild type and in treatment their is a value, so I am not able to calculate fold change and p...
15 December 2016 5,687 7 View
Hi all, is someone has idea how to calculate p value from RNA seq data if we have only one value for fold change. I have average of two replica not individual one so I have only one value. I need...
14 December 2016 8,596 6 View
hi, anyone please suggest me technique to detect presence of homodimer formation. I need to know homodimer formation of mouse nfkb1 which has mol wt of 50 Kda and when i am infecting raw cells...
28 September 2016 2,837 3 View
HI, I am working on nfkb1 and I need to check its expression on Raw and THP-1 cells by western as well I need to immunoprecipitation for its interacting partners. I have taken p50 ab from...
27 September 2016 7,097 3 View
Hi All, I need to perform double transfection in raw 264.7 with mouse p50-flag tag and his tag to know p50:p50 homodimer formation? I need to know is position of tag going to affect...
15 September 2016 2,715 1 View
I have mouse p50- c-terminal flag tag I PCDNA vector but I need two tags one with his as well, so it is important that his tag is also on C-terminal ad pcdna 3 only?? can I use pcdna3.1??I need to...
09 August 2016 1,708 1 View
Hello I'm looking for transcription factor resource that would give a list of transcription factors and their corresponding target gene. The species I'm mostly interested in are Mouse.
21 July 2016 8,948 0 View
hi, could anyone tell me database for pro-inflammatory and anti-inflammatory cytokines.
10 June 2016 7,991 4 View
can anyone suggest commercially available p50 *nfkb1)knock out any macrophage cell line particularly Raw264.7.
02 June 2016 1,895 1 View
I need to know hoe we can calculate how much induction of inos is there in treated compared to control with absorbance value. Hoe to use standard curve value in exp values??
12 May 2016 6,771 4 View
HI all, can anyone tells me if we want to infect any cell line with bacteria so can we seed cells up to maximum amount we can seed in an dish or less? like in six well i use to seed 1 million o/n...
08 May 2016 9,898 2 View
had anyone luck with this kit for NF-kb activation or this kit tells us something else?'
08 May 2016 5,661 0 View
i need to whats the exact difference as these are very related term and suppose i need to study effect of H.pylori(bacteria) on innate immune cell line such as -U937, thp-1 and raw etc, how we can...
04 May 2016 853 2 View
Hi,all.can anyone tell me how to Calculate fold change in RNA seq data if we have change from.zero to.something and something to zero in. Case of tanscription factor knock out mice .
02 May 2016 5,824 4 View
I need to know tf can bind to promoter or enhancer or both at a time?? is it a random process or specificity plays an important role?? is it generalizes for all tf, i need to know abt p50??
06 April 2016 5,589 1 View
