had anyone luck with this kit for NF-kb activation or this kit tells us something else?'
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...
02 March 2021 8,481 3 View
I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...
28 February 2021 3,637 2 View
Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...
24 February 2021 1,356 8 View
We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...
23 February 2021 1,898 1 View
Suggest me the method or some other software to calculate the above said data for nanoparticles.
21 February 2021 3,350 7 View
How to find the air side pressure drop in a spiral fin cross flow heat exchanger? The pressure difference at the inlet and outlet is very low compared to the experimental results, which I...
18 February 2021 1,109 2 View
Dear scholars, I was wondering if there are any scientific methods to train a neural network or ANFIS when one of the input variables is stochastic (random) and more importantly non-measurable?...
17 February 2021 1,909 3 View
I'm looking to do a sliding wear (plate-on-plate) simulation of GFRP composite plates with Archard Wear Model. Any hints or example steps are appreciated.
17 February 2021 1,447 2 View
There are numerous roots in this tap root system. Kindly suggest or share a research paper where I can clarify the doubts as to which root should be chosen for sectioning and for further root...
14 February 2021 1,816 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...
13 February 2021 7,217 4 View
Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...
11 February 2021 8,421 13 View
Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...
10 February 2021 5,863 2 View
I would like to evaluate the amount of reverse transcription (cDNA) on a 20um fixed mouse brain section. I am using random primers with RevertAid H Minus M-MuLV (reverse transcriptase). and would...
08 February 2021 1,957 4 View
I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...
05 February 2021 2,530 3 View
Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...
05 February 2021 292 3 View
I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...
05 February 2021 9,834 4 View
I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...
05 February 2021 4,159 1 View
Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...
05 February 2021 7,276 1 View