Hi,all.can anyone tell me how to Calculate fold change in RNA seq data if we have change from.zero to.something and something to zero in. Case of tanscription factor knock out mice .
The common approach is adding a very small number to all gene expressions. Let's say you add 1 or 0.001 (or anything similar) to all the numbers you have in the table. Then you do not have division by zero anymore and you can express FC. Be aware you create so called pseudo-counts since you don't work with the original data anymore.
The common approach is adding a very small number to all gene expressions. Let's say you add 1 or 0.001 (or anything similar) to all the numbers you have in the table. Then you do not have division by zero anymore and you can express FC. Be aware you create so called pseudo-counts since you don't work with the original data anymore.