I am trying to make Sabouraud Dextrose Agar for growing Candida albicans. I am using the following ingredients:
Peptone 10 grams
Dextrose: 40 grams
Agar: 15 grams
Distilled water added up to 1000 ml
I adjusted the pH to 5.6 using HCl.
I boiled the suspension to completely dissolve the contents.
Thereafter, I kept the media for autoclaving at 121O c for 30 minutes.
After autoclaving I found that the media does not get solidified even after 16 hours.
Please suggest me where is the problem.
Thanking you in advance.
Agar does not solidify in low pH, especially when the pH is adjusted before autoclaving. Try and see how much HCl you need for getting pH 5.6 and add it after autoclaving. Best of luck 👍
@ Shantanu, as Jaub suggested please add HCl after autoclaving to adjust the pH. Moreover, sometimes agar quality also not good, in that case you can increase your agar content from 15g to 20g per litre. Probably that may also help you for solidification.
Dear Shantanu Tamuly,
I recommend you check two things in the preparation process.
The first thing is to be sure that the agar gel totally melted during the boiling step. the Medium should become very transparent. This is the sign of a complete melt of the agar. The second thing is the quality of the agar. Be sure that you are using an agar of high quality (High grade) otherwise you need to increase the concentration up to 20g/L.
Hope it might help.
Good Luck.
First repeat the procedure with modification by increasing the amount of sgar_ agar added ,then pH adjusted after autoclave. With best regard
See the agar used is fresh.
Sterilize at 121 degree C for 15 minutes.
pH adjustment not usually done.
Learn the preparation of SDA .
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Infectious Diseases