I am trying to quantify the PEG density, coverage and finally the conformation of PEG grafted on a metallic nanoparticle coated with a thin layer of Silica. The PEG I am using is a Silane-PEG-NHS. Is there any method of reacting the NHS group of the PEG with a fluorescent dye (after grafting), so that I can measure the fluorescence by UV-Vis spectroscopy to quantify the PEG density/ coverage.

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