I am working with NIR excitable nanoparticles on in-vivo tumor model. Does anyone know a safe laser power/ dose at the NIR (980 nm) range, that does not cause skin burn in mouse. What is the maximum temperature that mouse skin could withstand before causing any molecular changes due to hyperthermia.
I think that, before that, you should care about dye photobleaching and autofluorescence effects, that I think would happen before observing thermal problems, leading to bad readings, and wrong quantification of NP concentration.
I wouldn't exceed 200 microwatt laser power for more than 1 seconds of integration time, as good measure.
it depends on skin color, thickness of the skin , sex and type of breed etc.
from my laser tissue welding studies experience I can tell you that it will be between 75 to 300 mW or even higher average power CW laser that depends on scan speed and dwell time if you are using robotic device to scan the surface
please refer my article that may be of some help to you... Best wishes
Laser tissue welding analyzed using fluorescence, Stokes shift spectroscopy, and Huang‐Rhys parameter
Journal of Biophotonics
Vidyasagar Sriramoju Robert R. Alfano
DOI: 10.1002/jbio.201100050
http://onlinelibrary.wiley.com/doi/10.1002/jbio.201100050/abstract
Also refer
Management of heat in laser tissue welding using NIR cover window material
Lasers in Surgery and Medicine
Vidyasagar Sriramoju Robert R. Alfano
DOI: 10.1002/lsm.21143
http://onlinelibrary.wiley.com/doi/10.1002/lsm.21143/abstract
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