Sasha Nealand

11 Questions 8 Answers 0 Followers

Questions related from Sasha Nealand

What does RV mean in the designation of a protein or gene of mycobacteria? For instance Rv2748c?

Where does this RV followed by a number come from?

17 August 2023 336 3 View

Bruker TOPSIN software green and blue contour, what does it tell me?

What information do the blue(positive contour) and green(negative contour) tell me in HSQC? Can I discover any information about a structure depending on if the contour is negative or positive?

17 August 2022 7,933 5 View

In NMR is H,C COSY the same as HSQC?

was reading an older textbook (Duddeck) they describe H,C correlated COSY, interpreted as C connected to H. Is this essentially interpreted the same as HSQC and HETCOR?

11 July 2022 8,122 3 View

Can plant material extracted with ethyl acetate be safely ingested?

If I have extracted a plant with ethyl acetate and found that this extract has an effective activity, is it generally safe for humans to ingest this extracted plant material? Ethyl acetate is GRAS...

07 April 2021 507 6 View

Can a used MilliQ water filter be successfully back flushed or flushed with solvent and reused?

Wondering if anyone has done this successfully? Thinking of backflushing and perhaps running methanol through the MilliQ filter column to clean it so it can be reused.

13 January 2021 5,885 0 View

Using p-anisaldehyde-H2SO4 TLC stain to detect terpenes, what compounds do the different colors indicate?

is it true that monoterpene alcohols are blue and esters are violet? what compounds do red green and yellow spots indicate?

27 April 2020 9,815 3 View

Our instrument room at my Uni does not have the money to maintain its instruments, anyone know of any good grants for this?

How can students find enough money to fund the analytical instrument room (LC/MS, NMR, GC/MS) at their University so that they can can continue their doctoral research?

18 December 2019 617 3 View

Is there any problem with using 0.1%TFA in HPLC mobile phase if the purpose is isolation, structure elucidation by NMR and bioassay?

20 July 2019 4,791 1 View

Simple recrystallization method for obtaining pure compound (natural product)?

Can anyone explain to me a simple procedure and solvent that would help me to obtain pure crystals? I am trying to isolate a pure compound that came off a silica column it was a solvent mixture of...

20 October 2018 403 5 View

Is it possible to use hexane:ethyl acetate mobile phase on a reverse phase column?

I am reading a journal paper in which separation of a plant extract was done on a C18 HPLC column using normal phase solvents of hexane:ethyl acetate, is this possible?

25 July 2018 2,412 13 View

How to dissolve insoluble sample in methanol or acetonitrile for LC/MS analysis?

I separated a crude plant extract into fractions on a silica column using ethyl acetate and hexane, now I want to look at the fractions using LC/MS. The problem is that our instrument room only...

25 July 2018 4,246 6 View