Our instrument room at my Uni does not have the money to maintain its instruments, anyone know of any good grants for this?

How can students find enough money to fund the analytical instrument room (LC/MS, NMR, GC/MS) at their University so that they can can continue their doctoral research?

17 December 2019 563 3 View

What are the specifications for centrifuging reagents prior to ELISA?

I'm going to perform an ELISA for fish GnRH (using CUSABIO). The "Technical Tips" pamphlet that came with the instructions manual says I need to centrifuge all reagents before use "as to...

17 September 2019 5,627 2 View

Is there any problem with using 0.1%TFA in HPLC mobile phase if the purpose is isolation, structure elucidation by NMR and bioassay?

Is there any problem with using 0.1%TFA in HPLC mobile phase if the purpose is isolation, structure elucidation by NMR and bioassay?

19 July 2019 4,749 1 View

Is it possible to use hexane:ethyl acetate mobile phase on a reverse phase column?

I am reading a journal paper in which separation of a plant extract was done on a C18 HPLC column using normal phase solvents of hexane:ethyl acetate, is this possible?

24 July 2018 2,368 13 View

How to dissolve insoluble sample in methanol or acetonitrile for LC/MS analysis?

I separated a crude plant extract into fractions on a silica column using ethyl acetate and hexane, now I want to look at the fractions using LC/MS. The problem is that our instrument room only...

24 July 2018 4,146 6 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View