Using p-anisaldehyde-H2SO4 TLC stain to detect terpenes, what compounds do the different colors indicate?

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Problems with RoB2 Excel tool - unable to generate overall risk of bias assessment?

Hi all, I am using the RoB2 tool on Excel for the first time today, and have been faced with some issues. When filling in each domain, the algorithm has successfully calculated the domain risk...

28 January 2024 5,281 1 View

What does RV mean in the designation of a protein or gene of mycobacteria? For instance Rv2748c?

Where does this RV followed by a number come from?

16 August 2023 3,443 3 View

What does RV mean in the designation of a protein or gene of mycobacteria? For instance Rv2748c?

Where does this RV followed by a number come from?

16 August 2023 374 3 View

I have run siRNA transfection on oesophageal cancer cells (SNO) & incubated for 48 hours but did not pick up any sign of knockdown of my target gene ?

I have run siRNA transfection on SNO cell lines. I seeded in a 6 well plate, incubated at 24 hours and treated with different volumes and concentrations of the siRNA and transfection reagent. I...

29 June 2023 5,439 1 View

Does anyone need a researcher for a meta-analysis/ systematic review?

I am a 3rd medical student and I'm interested in working on a meta-analysis/ systematic review, however, I am a fledgling when it comes to this realm of research. I have published an LTE and I'm...

11 May 2023 5,860 4 View

Environmental enrichment in shrimp / crustaceans?

Hi, Do you have recommendations of any articles on EE in shrimp / crustaceans. If there are any published studies out there it will be limited. Unfortunately all the info I have come across...

05 February 2023 562 5 View

Dry versus wet electrical stunning in crustaceans, the pros and cons of each?

Hi, I am looking for papers on electrical stunning in crustaceans where the pros and cons of dry versus wet stunning is discussed / described. Thanks.

07 December 2022 117 3 View

Bruker TOPSIN software green and blue contour, what does it tell me?

What information do the blue(positive contour) and green(negative contour) tell me in HSQC? Can I discover any information about a structure depending on if the contour is negative or positive?

16 August 2022 7,969 5 View

In NMR is H,C COSY the same as HSQC?

was reading an older textbook (Duddeck) they describe H,C correlated COSY, interpreted as C connected to H. Is this essentially interpreted the same as HSQC and HETCOR?

10 July 2022 8,159 3 View

Can plant material extracted with ethyl acetate be safely ingested?

If I have extracted a plant with ethyl acetate and found that this extract has an effective activity, is it generally safe for humans to ingest this extracted plant material? Ethyl acetate is GRAS...

06 April 2021 550 6 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How can I measure the thickness of thin film by Uv-Vis?

06 August 2024 7,810 3 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View

How to improve peaks separation in HPLC?

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30 July 2024 435 3 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

Lassaad Hedhili

Interesting question

Riaz A. Khan

Check the book by Egon Stahl on Chromatography titled Chromatography!

Frank T. Edelmann

Dear Sasha Nealand, for more information on this interesting question please check out the following previous discussion on RG:

https://www.researchgate.net/post/How_does_anisaldehyde_sulphuric_acid_reagent_yield_different_colours_in_TLC_during_derivatisation

P.S. I wish I could work at the UH at Hilo! 😎🌴