Our instrument room at my Uni does not have the money to maintain its instruments, anyone know of any good grants for this?

How can students find enough money to fund the analytical instrument room (LC/MS, NMR, GC/MS) at their University so that they can can continue their doctoral research?

17 December 2019 563 3 View

What are the specifications for centrifuging reagents prior to ELISA?

I'm going to perform an ELISA for fish GnRH (using CUSABIO). The "Technical Tips" pamphlet that came with the instructions manual says I need to centrifuge all reagents before use "as to...

17 September 2019 5,627 2 View

Is there any problem with using 0.1%TFA in HPLC mobile phase if the purpose is isolation, structure elucidation by NMR and bioassay?

Is there any problem with using 0.1%TFA in HPLC mobile phase if the purpose is isolation, structure elucidation by NMR and bioassay?

19 July 2019 4,749 1 View

Is it possible to use hexane:ethyl acetate mobile phase on a reverse phase column?

I am reading a journal paper in which separation of a plant extract was done on a C18 HPLC column using normal phase solvents of hexane:ethyl acetate, is this possible?

24 July 2018 2,368 13 View

How to dissolve insoluble sample in methanol or acetonitrile for LC/MS analysis?

I separated a crude plant extract into fractions on a silica column using ethyl acetate and hexane, now I want to look at the fractions using LC/MS. The problem is that our instrument room only...

24 July 2018 4,146 6 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View