Our instrument room at my Uni does not have the money to maintain its instruments, anyone know of any good grants for this?

How can students find enough money to fund the analytical instrument room (LC/MS, NMR, GC/MS) at their University so that they can can continue their doctoral research?

17 December 2019 563 3 View

What are the specifications for centrifuging reagents prior to ELISA?

I'm going to perform an ELISA for fish GnRH (using CUSABIO). The "Technical Tips" pamphlet that came with the instructions manual says I need to centrifuge all reagents before use "as to...

17 September 2019 5,627 2 View

Is there any problem with using 0.1%TFA in HPLC mobile phase if the purpose is isolation, structure elucidation by NMR and bioassay?

Is there any problem with using 0.1%TFA in HPLC mobile phase if the purpose is isolation, structure elucidation by NMR and bioassay?

19 July 2019 4,749 1 View

Is it possible to use hexane:ethyl acetate mobile phase on a reverse phase column?

I am reading a journal paper in which separation of a plant extract was done on a C18 HPLC column using normal phase solvents of hexane:ethyl acetate, is this possible?

24 July 2018 2,368 13 View

How to dissolve insoluble sample in methanol or acetonitrile for LC/MS analysis?

I separated a crude plant extract into fractions on a silica column using ethyl acetate and hexane, now I want to look at the fractions using LC/MS. The problem is that our instrument room only...

24 July 2018 4,146 6 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View