Hi everyone

I want to introduce some iron stress to haloferax cells in the culture.

Normally, i have been applying low iron stress where i just prepare media with low iron (Hv.Min minus 23 mg FeSO 4 x 7H2O).

Now i want to apply high iron stress, what is the criteria for increasing or decreasing any specific nutrient in the media??

Earlier i just followed previously established protocol in the lab so now I want to know how does it work.

Thanks in advance.

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