Hi everyone
I want to introduce some iron stress to haloferax cells in the culture.
Normally, i have been applying low iron stress where i just prepare media with low iron (Hv.Min minus 23 mg FeSO 4 x 7H2O).
Now i want to apply high iron stress, what is the criteria for increasing or decreasing any specific nutrient in the media??
Earlier i just followed previously established protocol in the lab so now I want to know how does it work.
Thanks in advance.