There are problems germinating old transgenic rice seeds (Japonica). Some seeds are 4 years old, while others are 3. They were stored at room temperature. Can we treat them in a way to have at least 20-30% germinate? Please let me know.
Thanks
Dear Sachin Teotia
I think the first thing you should do is to select within those rice samples the best kernels for germination. There is a very easy and effective method proposed by Japan International Cooperation Agency, which I add in the links below:
- Method summarized ( http://www.rkmp.co.in/content/selection-of-seed-by-salt-water-treatment)
- JICA report. I recommend to readi it, in particular, epigraph 6.3 ( chrome-extension://oemmndcbldboiebfnladdacbdfmadadm/https://www.jica.go.jp/nepal/english/office/others/c8h0vm0000bjww96-att/tm_5.pdf )
I hope that this information can be useful for you,
All the best
Go for seed priming @15 0C, for 24 hours. This will lead to repair of damage due to seed ageing. Refer https://www.researchgate.net/project/STUDIES-ON-APPLICATION-OF-POLYMER-SPERMINE-AND-SCAPE-REGULATION-ON-SEED-YIELD-AND-QUALITY-IN-ONION-Allium-cepa-L-AND-GARLIC-Allium-sativum-L for further details. You can expect 30-40 % higher germination compared to control. Alternatively you can go for tetrazolium test to accertain if the seeds are not dead.
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