I am regenerating transformed rice calli on MS-medium. Before this, all stages were grown in the dark. But after keeping them in light for regeneration, a lot of condensation has formed on the lid of the petriplates. I think this condensation, in the form of water droplets, will get bigger with time and might fall on the calli. How to prevent it? The temperature of the incubation area is about 29oC and light intensity is about 200 micromol/m2/sec. And this is somewhat recommended. There was slight condensation even in the dark with the temperature of about 26-27oC. I think light is doing it, but then we need light for regeneration. How to proceed? Thanks!!
My supervisor advised me to place some (1-3) empty petriplates on the plates you have callus or regerenating calli. If you are using disposible petriplates replaced the lid with fresh one. and place some empty petriplates on it in the growth room. it results better
Poor you that you have to have such high temperatures! But even with calli at 25 C and light of 30 micro E there is condensation on the lid. Normally I put the dishes with bottom up. Or if you have already some small shoots, transfer them to tubes, then seal with parafilm and you are rid of condensation and the worry of fungus developing.
Hi Sachin,
Dry your media plates for longer time and then use them.
Yes, that do other labs too. To leave half oben the petridishes for at least 30 min in the hood and then close them. But they told me that they still had condensation when using even these plates in 25 C and under light.. So maybe really you try to grow the callus in tubes.
They are some practices to reduce the condensation problem. The expensive way is to use the bottom cooling system, that cools down the temperature of bottom part of tissue culture container. The cheap way is to place a layer of insulation material on the shelf, bette with piece of alluminum plate on top. It also works although no as good as the expansive way.
It is better to leave the plates for 30 min half-open after pouring the hot medium in the plates. This reduces accumulation of moisture inside the lid. I follow this method and never had this problem.
I love this question, I had similar problem last year while transforming rice but not anymore.
DO NOT PLACE YOUR PLATES ON FLAT SURFACE, instead use Mesh or fridge Rack that permits light to pass through. Secondly, control the humidity of the growth room/incubator (if you can do that). Temperature is NOT the cause of condensation. I used different range of Temperature from 32 to 25oC under the two condition and I found out that Temperature does not cause the condensation.
I hope this help.
I had this problem a lot, and the only way to fix it is avoid use shelves that have lights turned on below. Usually I put my petri dishes just in the last shelf or in another space that don't have lights turned on. Leave the media culture dry for more time will not resolve your problem. To optimize space on the shelves I alternate them with magenta boxes with plants already formed because there is no problem with condensation. Another option but sometimes does not works is put yours plates in up side down position.
Hi Sachin,
Excess of condensation is normal especially when you have power light bulbs...can you find a chamber with cool-white bulbs/tubes? Those cool-white lamps irradiate less heat. Your problem with excess of condensation (again, condensation is rather normal but over-condensation should be avoided) is from the heat that is irradiated from the light tubes/bulbs. I subscribe the use of styrofoam under the petridishes. That will help and also drying the plates a bit longer...
Hi Sachin,
1. For several years, several labs and ours in the same building have used medical gas-permeable tape (Micropore™ Surgical Tape; 3M Health Care, USA) to wrap the plant tissue-culture plates, instead of using parafilm (see below attached pictures). This reduces the condensation on plate lids.
2. I agree with Denise, in a walk-in growth chamber, I avoid use the shelves that have lights turned on below. Normally, I also choose the lowest shelf to place my plates, which have no light bulbs hanging below. I see rare to no condensation, compared to those plates placed on the upper shelves.
it is critical to dry the medium before transferring calli on the plate.
1. We use a separate rack with mesh of 4-5" height on the racks to avoid heat from the lower rack lights.
2. The porous tape above mentioned not only reduces the condensation excellently but improves the regeneration greatly. However, the medium dries up very fast so pour the medium almost to the brim with 1-2 mm gap so after two weeks enough medium will be available to sustain the the callus regeneration.
Dear Sachin.
Put some recipients with water near to the petri plates. Your problem is humidity. I suggest not using petri dishes (in future work) replace them with glass bottles, this problem can be solved easier with these containers.
Dear Sachin Teotia
Pl. prepare a cone pyramid shape plastic transparent jar according to your requirement. Place t he petri dishes across its interior walls but without lid. Leave the central space quite under the cone free. Place at this point dry lab scale Silica, or butter paper, or sponge or glass wool or cloth or any such available material capable of collecting the condense vapours. Opimize the space and jar according to numbe rof petri dishes.
share results.
simple thing, you try keeping the plates in inverted position to remove the condensed water.
Condensation will not occur in inverted plates...
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Enzymology
- Enzymes