1. Prepare minimal media for PHA-producing bacterial cells and add 10 microgram/mL DMSO in the media.
2. Inoculate a loopful of culture in the media and incubate for 48-72 hours.
3. After the incubation period, take a loopful of culture and prepare a smear on a clean glass slide. Air dry the slide and visualise under the fluorescence microscope.