Can someone share standard protocol for DNS test of glucose?

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How to find out or construct(?) the right priors from data of previous experiment to use in a bayesian glm?

I have the following equation, Eating choice (Yes/No)~ Box type (A/B/C). So the question is whether a certain box type affects eating choice in groups of dogs. The eating choice of each member in...

10 February 2021 6,024 9 View

How can a publish my case report?

I want to publish a case report regarding jejunal diverticula

02 January 2021 7,630 5 View

How to calculate the dimensions and frequency, etc. of a pulse tube for required cold/hot temperatures?

Require to know the methodology for calculating dimensions (Lenght, diameter), operating frequency, etc. of a simple pulse tube refrigerator. Reference to handbook or manuscript would also be helpful.

29 September 2020 2,191 2 View

Is there any cost-effective, simple and efficient way to analyse and estimate the amount of vitamins in algal samples?

I am currently pursuing my research in Phycology. I'd like to know if there's any simple and cost-effective method to detect and analyze nutrients and vitamins in the algal matter. The experiment...

18 September 2020 4,081 3 View

Which research institutes in India have an algal identification facility?

I am currently pursuing my research in Phycology and planning to conduct a study on microalgae. Is there any place where I can send my samples to be identified? (Preferably in South India) Also,...

17 September 2020 4,440 2 View

Reaching out about helping in empirical research!?

Dear all, It is a kind and humble request to fill the following survey questionnaire. Your cooperation is highly appreciated in this regard. It will not take more than 10 minutes of your time. The...

31 August 2020 5,650 5 View

Could anyone please summarise in short about what Kalman filter is? Or suggest a good article for that? Can it be used to get DC value of a signal?

I want to understand the overview of Kalman filters and what purpose do they serve. I am trying to leverage this platform as I am not sure about other authentic sources on the internet to get this...

09 August 2020 7,440 4 View

Ideal qualities of a researcher: Let's share our opinions?

Opening a debate on the qualities of an ideal researcher...

24 July 2020 9,819 5 View

Retractions in publishing (often found across journal issues now): Discussing the reasons, journal policies, consequences n mitigation strategies?

Retraction is a pain in publishing: Opening a debate on this ever-evolving and a topic of wide concern.

24 July 2020 1,386 11 View

How can I study the microstructure of carbon steel C1010 by etching process?

How can I study the microstructure of carbon steel C1010 by etching process. Which chemicals I should use for etching of carbon steel?

07 July 2020 5,106 7 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

How to simulate enzymatic hydrolyis of sucrose in a CSTR coupled with ultrafiltration membrane with comsol?

Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...

27 January 2021 2,525 4 View

About protocols used for glucose uptake in HepG2 cells?

Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...

25 January 2021 7,608 3 View

How to measure Tg (Glass Transition) in lyophilised sugar/protein matrices using DSC?

I have been attempting to measure the glass transition temperature of various amorphous powders consisting of sugar/protein mixtures and have had little luck. I typically use a scanning rate of...

13 January 2021 10,022 3 View

How long does Hela cancer cells take to reach 80% confluency after thawing from liquid nitrogen?

Three days ago, i thawed Hela cancer cells from liquid nitrogen but until now they are still suspended in the media and very very low number of cells became attached to the tissue culture...

06 January 2021 6,511 4 View

What's the function of each ingredient in a binding buffer?

I read a binding buffer in references. The recipe was listed as follows: Glucose (4.5g/L), Magnesium Chloride (5 mM), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml) was dissolved in DPBS. This buffer...

23 December 2020 8,440 4 View

Aref Wazwaz

Dear Dr Rohan Samir Kumar Sachan . See the following useful link: https://user.eng.umd.edu/~nsw/ench485/lab4a.htm

Kindly see also the following useful RG link: Article Evaluation of the DNS method for analysing lignocellulosic h...

Also I hope the following RG link is useful: Chapter Quantitative Analysis of Reducing Sugars by 3, 5-Dinitrosali...

Rohan Samir Kumar Sachan

Thank you sir and ma’am for your help.