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- used for making matrices to seed neonatal fibroblasts.

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Starch analysis - Ewers is 10520 method - elevated starch results?

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Functional/non specific disulphide bond formation in proteins?

As an alternative to looking at the conservation of cysteine residues amongst homologs of a protein of interest, is anyone aware of a server that can predict whether disulphide bond formation...

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Protein precipitates on purification??

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20 March 2024 9,095 4 View

Multiple bands detected in SDS sample of monomer species (gel filtration fraction)??

I have purified several his tagged proteins using IMAC. To clean up the samples further I ran them through a GF column (Superdex 200 increase 10/30Gl). When I ran an SDS sample containing the...

19 March 2024 4,202 3 View

Colorimetric glycoalkaloids in potato?

Hi, Has anyone got a method to test glycoalkaloids in potato. I don't have an HPLC so I'm looking for a wet chemistry method. I would like to quantify the results. If possible, I want to look a...

13 November 2023 1,668 1 View

Peroxide value and free fatty acid control?

We use an I house sample as a control sample (set up by running a PT sample along side it). However, over a few months it becomes rancid and we have to start the process again. Does anyone know of...

27 July 2023 8,679 0 View

Does anyone know of any research around a recursive learning cycle that promotes contextual learning and flexible thinking in Elementary Education?

Currently exploring similarities around the Design Thinking, Inquiry Learning, and Mathematical Modelling framework.

17 May 2023 8,746 1 View

Best way to inflate the pancreas for mouse islet isolation?

as stated

18 February 2023 7,264 1 View

Binding affinity (KD) of DNA oligos for their complement?

Folks, Does anyone know of a paper showing binding affinity data of DNA oligos for their complement? Ideally it would show data from a 'non-molecular biology method' such as ITC, SPR, or MST. Even...

12 January 2023 2,965 2 View

Can I still use a chi-square/ Fisher's exact test if the following two conditions are not met?

Can you use the chi-squared test if subjects from the groups in the predictor variable can appear multiple times in the response variable categories? & Can you use chi-square if you do not...

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