Can I use GC-MS instead of LC-MS for confirmation of synthesized benzyl mercaptan adducts (catechin-BM, Epicatechin-BM, Gallocatechin-BM, and Epigallocatechin-BM)?
I can't seem to find methods utilizing gc-ms.
I have not analysed this groups of compounds myself, but I would think GC-MS is an option.
It might need derivatising (methylating or sililating) the thiol group to get that more stable.
Not volatile, GC-MS will mean an additional derivatisation step, with all the attendant problems. Like cleavage of the binding between flavan-3-ol and benzyl group, degradation of the polyphenols etc. And a lot of work from your side to establish the best deriatisation plus its precise conditions. Not a good idea.
Hi Catherine M G C Renard
there are studies that have confirmed what thioethers are produced when certain methods are done (Gea et al., 2011; Kolodziej et al., 1990; & Steynberg et al., 1998). Do you think that is enough for an undergraduate to cite? We just really don't have access to LC-MS in our lab.
No, GC is for the analysis of volatile compounds only. As Catherine notes, the compounds would require modification to make them suitable. The chemical changes needed would probably introduce addition sources of error, wasted time and ignore doing things the simplest and most reliable way. A sample's properties often dictate which analysis method(s) are appropriate to use. Use the appropriate scientific technique. If it is not available to you, send it to an outside lab that has the expertise and instrumentation needed.
you can use hydrophobic interaction chromatography (HILIC), supercritical fluid chromatography (SFC), size-exclusion chromatography (SEC), ion-exchange chromatography (IEC), and hydrophobic interaction chromatography (HIC)
As non-volatile compounds, their direct analysis by GC-MS is not possible. You can do it after derivatisation, however, the work associated to this step (optimal conditions) is too hard.
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