I want to find out different isoforms of a gene. I know microarray or RNA seq can be used. But I need clarification or any other methods that can be used.
Libor Krásný
Microarrays may not have sufficient resolution. RNAseq is better. Ideally, Oxford nanopore sequencing of RNA could be used to directly detect transcript variants. Validation could be done with Northern blot analysis.
0 votes
0 thanks
Anita Nayal
RNA-Seq (Best method) – Provides high-resolution transcript isoform identification and quantification. Microarray – Not ideal, as it mainly detects known transcripts and lacks isoform-level resolution. qRT-PCR – Can validate specific isoforms if primers are designed accordingly. Isoform-Specific Databases – Use resources like Ensembl, RefSeq, or GENCODE for known isoforms.
According to me,
To find transcript isoforms of a gene, you can use:
Microarrays are not suitable for discovering novel isoforms but can be used to detect known ones. RNA-Seq is the preferred method.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Computer Communications (Networks)
- Simulators
More Payel Das's questions See All
Similar questions and discussions