How much (chloroform: n-butanol = 4:1) can be used for deproteinization of polysaccharide solution?

29 October 2019 1 4K Report

I am using the Sevag method to the purification of the EPS to remove protein but I don't know how much sevag agent (chloroform: n-butanol = 4:1) should I use?

Paria Rahnama

Majid Nooshkam

Thank you for your help

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