Hello,
I would like ask somebody for infromation about protocol for apoptotic sperm cells staining, using Yo-Pro 1 probe and PI. I got protocol from one laboratory, where they use PI concentration only 5 micrograms of PI per 1 ml of physiological saline. When I was trying this concentration, the pattern of dead sperms red/green was not be possible to detect under fluorescent microscope.
Thank you