Dear pl refer to this link http://www.reproduction-online.org/content/89/1/127.full.pdf, wherein 50 micro molar calcium ionophore A23187 has been used for this purpose. But, again you will have to follow and optimise your protocol accordingly. Good luck!

According to the 2010 WHO guidelines you should use a final concentration of 10umol/L.

Remember that you must first allow the sperm to capacitate for 3 hours.

Thereafter you need to stimulate for 15 minutes at 37C with the A23187

5uM would be enough to avoid DMSO solvent toxicity.

But it all depends on the conc. and the duration of treatment. There are so many papers. Try Google!!!

You can initially make a stock solution at 5 mM in DMSO to dilute to 10 μM at the moment of use.

Dear all,

thank you for your suggestions.

My best regards,

Ondřej